SMAD2 Protein (AA 2-467) (His tag)
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- 抗原 See all SMAD2 蛋白
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-467
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宿主
- 人
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资源
- Insect Cells
- 标记
- This SMAD2 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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SSILPFTPPV VKRLLGWKKS AGGSGGAGGG EQNGQEEKWC EKAVKSLVKK LKKTGRLDEL EKAITTQNCN TKCVTIPSTC SEIWGLSTPN TIDQWDTTGL YSFSEQTRSL DGRLQVSHRK GLPHVIYCRL WRWPDLHSHH ELKAIENCEY AFNLKKDEVC VNPYHYQRVE TPVLPPVLVP RHTEILTELP PLDDYTHSIP ENTNFPAGIE PQSNYIPETP PPGYISEDGE TSDQQLNQSM DTGSPAELSP TTLSPVNHSL DLQPVTYSEP AFWCSIAYYE LNQRVGETFH ASQPSLTVDG FTDPSNSERF CLGLLSNVNR NATVEMTRRH IGRGVRLYYI GGEVFAECLS DSAIFVQSPN CNQRYGWHPA TVCKIPPGCN LKIFNNQEFA ALLAQSVNQG FEAVYQLTRM CTIRMSFVKG WGAEYRRQTV TSTPCWIELH LNGPLQWLDK VLTQMGSPSV RCSSMS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SMAD2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SMAD2 蛋白
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SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) protein (GST tag)
SMAD2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) protein (His tag)SMAD2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 1-467) protein (His tag)SMAD2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
SMAD, Mothers Against DPP Homolog 2 (SMAD2) protein (GST tag,His tag)SMAD2 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) protein (His tag)Crystallography grade SMAD2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
SMAD, Mothers Against DPP Homolog 2 (SMAD2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SMAD2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467), (full length) protein (His tag)SMAD2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % SDS, ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- 别名
- SMAD2 (SMAD2 产品)
- 别名
- JV18 Protein, JV18-1 Protein, MADH2 Protein, MADR2 Protein, hMAD-2 Protein, hSMAD2 Protein, jv18 Protein, Xmad2 Protein, madh2 Protein, madr2 Protein, XSmad2 Protein, hmad-2 Protein, hsmad2 Protein, jv18-1 Protein, smad-2 Protein, fj43c06 Protein, wu:fj43c06 Protein, 7120426M23Rik Protein, Madh2 Protein, Madr2 Protein, Smad-2 Protein, mMad2 Protein, MAD homolog 2 Protein, Mothers against DPP homolog 2 Protein, SMAD 2 Protein, Smad2 Protein, SMAD family member 2 Protein, SMAD family member 2-Z Protein, SMAD family member 2 S homeolog Protein, SMAD2 Protein, SMAD2Z Protein, smad2 Protein, smad2.S Protein, Smad2 Protein
- 背景
- Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. {ECO:0000269|PubMed:16751101, ECO:0000269|PubMed:16862174, ECO:0000269|PubMed:17327236, ECO:0000269|PubMed:19289081, ECO:0000269|PubMed:9892009}.
- 分子量
- 53.1 kDa Including tag.
- UniProt
- Q15796
- 途径
- Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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