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RPSA/Laminin Receptor Protein (AA 2-295) (His tag)

Crystallography grade RPSA 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
产品编号 ABIN3095138
发货至: 中国
  • 抗原 See all RPSA/Laminin Receptor (RPSA) 蛋白
    RPSA/Laminin Receptor (RPSA) (Ribosomal Protein SA (RPSA))
    蛋白类型
    Recombinant
    产品特性
    AA 2-295
    宿主
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    资源
    • 18
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    大肠杆菌(E. Coli)
    标记
    This RPSA/Laminin Receptor protein is labelled with His tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    序列
    SGALDVLQMK EEDVLKFLAA GTHLGGTNLD FQMEQYIYKR KSDGIYIINL KRTWEKLLLA ARAIVAIENP ADVSVISSRN TGQRAVLKFA AATGATPIAG RFTPGTFTNQ IQAAFREPRL LVVTDPRADH QPLTEASYVN LPTIALCNTD SPLRYVDIAI PCNNKGAHSV GLMWWMLARE VLRMRGTISR EHPWEVMPDL YFYRDPEEIE KEEQAAAEKA VTKEEFQGEW TAPAPEFTAT QPEVADWSEG VQVPSVPIQQ FPTEDWSAQP ATEDWSAAPT AQATEWVGAT TDWS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    产品特性
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RPSA Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    过滤
    0.22 μm filtered
    内毒素水平
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    质量等级
    Crystallography grade
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    说明

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    RPSA/Laminin Receptor (RPSA) (Ribosomal Protein SA (RPSA))
    别名
    RPSA (RPSA 产品)
    别名
    37LRP Protein, 67LR Protein, LBP/p40 Protein, LRP/LR Protein, LamR Protein, rpsa Protein, LamR1 Protein, RPSA Protein, LAMBR Protein, LAMR1 Protein, LBP Protein, LRP Protein, NEM/1CHD4 Protein, SA Protein, lamR Protein, p40 Protein, 67kDa Protein, 67lr Protein, AL022858 Protein, Lamr Protein, Lamr1 Protein, Lamrl1 Protein, MLR Protein, P40 Protein, P40-3 Protein, P40-8 Protein, lamr1 Protein, zgc:55831 Protein, zgc:77824 Protein, DMRT1 Protein, 40S ribosomal protein SA Protein, 67kD laminin receptor Protein, ribosomal protein SA Protein, rssa Protein, rpsa Protein, RPSA Protein, Rpsa Protein, LOC100144340 Protein
    背景
    Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. {ECO:0000255|HAMAP-Rule:MF_03016, ECO:0000269|PubMed:16263087, ECO:0000269|PubMed:6300843}., (Microbial infection) Acts as a receptor for the adeno-associated viruses 2,3,8 and 9, dengue virus, Sindbis virus and Venezuelan equine encephalitis virus (PubMed:1385835, PubMed:15507651, PubMed:16973587). Also acts as a receptor for pathogenic prion protein and bacteria (PubMed:9396609, PubMed:11689427, PubMed:15516338). {ECO:0000269|PubMed:11689427, ECO:0000269|PubMed:1385835, ECO:0000269|PubMed:15507651, ECO:0000269|PubMed:15516338, ECO:0000269|PubMed:16973587, ECO:0000269|PubMed:9396609}.
    分子量
    33.7 kDa Including tag.
    UniProt
    P08865
    途径
    Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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