MLH1 Protein (AA 2-756) (His tag)
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- 抗原 See all MLH1 蛋白
- MLH1 (MutL Homolog 1 (MLH1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-756
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宿主
- 人
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资源
- Insect Cells
- 标记
- This MLH1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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SFVAGVIRRL DETVVNRIAA GEVIQRPANA IKEMIENCLD AKSTSIQVIV KEGGLKLIQI QDNGTGIRKE DLDIVCERFT TSKLQSFEDL ASISTYGFRG EALASISHVA HVTITTKTAD GKCAYRASYS DGKLKAPPKP CAGNQGTQIT VEDLFYNIAT RRKALKNPSE EYGKILEVVG RYSVHNAGIS FSVKKQGETV ADVRTLPNAS TVDNIRSIFG NAVSRELIEI GCEDKTLAFK MNGYISNANY SVKKCIFLLF INHRLVESTS LRKAIETVYA AYLPKNTHPF LYLSLEISPQ NVDVNVHPTK HEVHFLHEES ILERVQQHIE SKLLGSNSSR MYFTQTLLPG LAGPSGEMVK STTSLTSSST SGSSDKVYAH QMVRTDSREQ KLDAFLQPLS KPLSSQPQAI VTEDKTDISS GRARQQDEEM LELPAPAEVA AKNQSLEGDT TKGTSEMSEK RGPTSSNPRK RHREDSDVEM VEDDSRKEMT AACTPRRRII NLTSVLSLQE EINEQGHEVL REMLHNHSFV GCVNPQWALA QHQTKLYLLN TTKLSEELFY QILIYDFANF GVLRLSEPAP LFDLAMLALD SPESGWTEED GPKEGLAEYI VEFLKKKAEM LADYFSLEID EEGNLIGLPL LIDNYVPPLE GLPIFILRLA TEVNWDEEKE CFESLSKECA MFYSIRKQYI SEESTLSGQQ SEVPGSIPNS WKWTVEHIVY KALRSHILPP KHFTEDGNIL QLANLPDLYK VFERC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MLH1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MLH1 蛋白
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MutL Homolog 1 (MLH1) (AA 407-756) protein (His tag)
MLH1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
MutL Homolog 1 (MLH1) (AA 647-756) protein (GST tag)MLH1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
MutL Homolog 1 (MLH1) (AA 1-756) protein (GST tag)MLH1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
MutL Homolog 1 (MLH1) (AA 2-760) protein (His tag)Crystallography grade MLH1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
MutL Homolog 1 (MLH1) protein (Myc-DYKDDDDK Tag)MLH1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MLH1 (MutL Homolog 1 (MLH1))
- 别名
- MLH1 (MLH1 产品)
- 别名
- CG11482 Protein, Dmel\\CG11482 Protein, dmlh-1 Protein, dmlh1 Protein, zgc:66301 Protein, MLH1 Protein, LOC100232198 Protein, 1110035C23Rik Protein, AI317206 Protein, AI325952 Protein, AI561766 Protein, COCA2 Protein, FCC2 Protein, HNPCC Protein, HNPCC2 Protein, hMLH1 Protein, mutL homolog 1 Protein, CG11482 gene product from transcript CG11482-RB Protein, mutL homolog 1 S homeolog Protein, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) Protein, DNA mismatch repair protein Mlh1 Protein, MLH1 Protein, Mlh1 Protein, mlh1.S Protein, mlh1 Protein, LOC588545 Protein
- 背景
- Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. {ECO:0000269|PubMed:16873062, ECO:0000269|PubMed:18206974}.
- 分子量
- 85.4 kDa Including tag.
- UniProt
- P40692
- 途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
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