CAMK2D Protein (AA 2-499) (His tag)
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- 抗原 See all CAMK2D 蛋白
- CAMK2D (Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-499
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宿主
- 人
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资源
- Insect Cells
- 标记
- This CAMK2D protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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ASTTTCTRFT DEYQLFEELG KGAFSVVRRC MKIPTGQEYA AKIINTKKLS ARDHQKLERE ARICRLLKHP NIVRLHDSIS EEGFHYLVFD LVTGGELFED IVAREYYSEA DASHCIQQIL ESVNHCHLNG IVHRDLKPEN LLLASKSKGA AVKLADFGLA IEVQGDQQAW FGFAGTPGYL SPEVLRKDPY GKPVDMWACG VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK MLTINPAKRI TASEALKHPW ICQRSTVASM MHRQETVDCL KKFNARRKLK GAILTTMLAT RNFSAAKSLL KKPDGVKEST ESSNTTIEDE DVKARKQEII KVTEQLIEAI NNGDFEAYTK ICDPGLTAFE PEALGNLVEG MDFHRFYFEN ALSKSNKPIH TIILNPHVHL VGDDAACIAY IRLTQYMDGS GMPKTMQSEE TRVWHRRDGK WQNVHFHRSG SPTVPIKPPC IPNGKENFSG GTSLWQNI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CAMK2D Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product CAMK2D 蛋白
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Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) (AA 1-478) (Active) protein (GST tag)
CAMK2D 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) (AA 2-115) protein (His-IF2DI Tag)CAMK2D 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) (AA 1-478) protein (GST tag)CAMK2D 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) (AA 301-410) protein (GST tag)CAMK2D 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) (AA 2-499) protein (His tag)Crystallography grade CAMK2D 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) protein (DYKDDDDK Tag)CAMK2D 宿主: 小鼠 宿主: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CAMK2D 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CAMK2D 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)CAMK2D 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- CAMK2D (Calcium/calmodulin-Dependent Protein Kinase II delta (CAMK2D))
- 别名
- CAMK2D (CAMK2D 产品)
- 别名
- CAMKD Protein, CAMK1 Protein, Camk2 Protein, Camki Protein, RATCAMKI Protein, camk2 Protein, camkd Protein, camkII Protein, 2810011D23Rik Protein, 8030469K03Rik Protein, [d]-CaMKII Protein, mKIAA4163 Protein, calcium/calmodulin dependent protein kinase II delta Protein, calcium/calmodulin-dependent protein kinase II delta Protein, calcium/calmodulin dependent protein kinase (CaM kinase) II delta S homeolog Protein, calcium/calmodulin dependent protein kinase (CaM kinase) II delta Protein, calcium/calmodulin-dependent protein kinase II, delta Protein, CAMK2D Protein, Camk2d Protein, camk2d.S Protein, camk2d Protein
- 背景
- Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor. {ECO:0000269|PubMed:16690701, ECO:0000269|PubMed:17179159}.
- 分子量
- 57.2 kDa Including tag.
- UniProt
- Q13557
- 途径
- WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration
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