FYN Protein (AA 2-537) (His tag)
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- 抗原 See all FYN 蛋白
- FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-537
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宿主
- 人
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资源
- Insect Cells
- 标记
- This FYN protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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GCVQCKDKEA TKLTEERDGS LNQSSGYRYG TDPTPQHYPS FGVTSIPNYN NFHAAGGQGL TVFGGVNSSS HTGTLRTRGG TGVTLFVALY DYEARTEDDL SFHKGEKFQI LNSSEGDWWE ARSLTTGETG YIPSNYVAPV DSIQAEEWYF GKLGRKDAER QLLSFGNPRG TFLIRESETT KGAYSLSIRD WDDMKGDHVK HYKIRKLDNG GYYITTRAQF ETLQQLVQHY SERAAGLCCR LVVPCHKGMP RLTDLSVKTK DVWEIPRESL QLIKRLGNGQ FGEVWMGTWN GNTKVAIKTL KPGTMSPESF LEEAQIMKKL KHDKLVQLYA VVSEEPIYIV TEYMNKGSLL DFLKDGEGRA LKLPNLVDMA AQVAAGMAYI ERMNYIHRDL RSANILVGNG LICKIADFGL ARLIEDNEYT ARQGAKFPIK WTAPEAALYG RFTIKSDVWS FGILLTELVT KGRVPYPGMN NREVLEQVER GYRMPCPQDC PISLHELMIH CWKKDPEERP TFEYLQSFLE DYFTATEPQY QPGENL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human FYN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product FYN 蛋白
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FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-482) protein (GST tag)
FYN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP, KAA
FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-537) (Active) protein (His-GST)FYN 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 41-190) protein (His-IF2DI Tag)FYN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 1-90) protein (GST tag)FYN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 2-537) protein (His tag)Crystallography grade FYN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
FYN Oncogene Related To SRC, FGR, YES (FYN) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)FYN 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))
- 别名
- FYN (FYN 产品)
- 别名
- im:6908851 Protein, si:dkey-119m7.5 Protein, zgc:109996 Protein, c-fyn Protein, cfyn-a Protein, SLK Protein, SYN Protein, p59-FYN Protein, PKC Protein, fyn Protein, zgc:86720 Protein, AI448320 Protein, AW552119 Protein, FYN proto-oncogene, Src family tyrosine kinase Protein, FYN proto-oncogene, Src family tyrosine kinase S homeolog Protein, FYN proto-oncogene, Src family tyrosine kinase b Protein, FYN proto-oncogene, Src family tyrosine kinase L homeolog Protein, FYN proto-oncogene, Src family tyrosine kinase a Protein, Fyn proto-oncogene Protein, FYN Protein, fyn.S Protein, fynb Protein, fyn.L Protein, Fyn Protein, fyna Protein
- 背景
- Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. {ECO:0000269|PubMed:11005864, ECO:0000269|PubMed:11162638, ECO:0000269|PubMed:11536198, ECO:0000269|PubMed:12788081, ECO:0000269|PubMed:14707117, ECO:0000269|PubMed:14761972, ECO:0000269|PubMed:15536091, ECO:0000269|PubMed:15557120, ECO:0000269|PubMed:16387660, ECO:0000269|PubMed:16841086, ECO:0000269|PubMed:17194753, ECO:0000269|PubMed:18056706, ECO:0000269|PubMed:18258597, ECO:0000269|PubMed:19179337, ECO:0000269|PubMed:19652227, ECO:0000269|PubMed:20100835, ECO:0000269|PubMed:22080863, ECO:0000269|PubMed:7568038, ECO:0000269|PubMed:7822789}.
- 分子量
- 61.6 kDa Including tag.
- UniProt
- P06241
- 途径
- JAK/STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling
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