Ephrin A1 Protein (EFNA1) (AA 1-1120) (Strep Tag)
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- 抗原 See all Ephrin A1 (EFNA1) 蛋白
- Ephrin A1 (EFNA1)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1120
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宿主
- 人
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This Ephrin A1 protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MVLNSLDKMI QLQKNTANIR NICVLAHVDH GKTTLADCLI SSNGIISSRL AGKLRYMDSR EDEQIRGITM KSSAISLHYA TGNEEYLINL IDSPGHVDFS SEVSTAVRIC DGCIIVVDAV EGVCPQTQAV LRQAWLENIR PVLVINKIDR LIVELKFTPQ EAYSHLKNIL EQINALTGTL FTSKVLEERA ERETESQVNP NSEQGEQVYD WSTGLEDTDD SHLYFSPEQG NVVFTSAIDG WGFGIEHFAR IYSQKIGIKK EVLMKTLWGD YYINMKAKKI MKGDQAKGKK PLFVQLILEN IWSLYDAVLK KDKDKIDKIV TSLGLKIGAR EARHSDPKVQ INAICSQWLP ISHAVLAMVC QKLPSPLDIT AERVERLMCT GSQTFDSFPP ETQALKAAFM KCGSEDTAPV IIFVSKMFAV DAKALPQNKP RPLTQEEIAQ RRERARQRHA EKLAAAQGQA PLEPTQDGSA IETCPKGEEP RGDEQQVESM TPKPVLQEEN NQESFIAFAR VFSGVARRGK KIFVLGPKYS PLEFLRRVPL GFSAPPDGLP QVPHMAYCAL ENLYLLMGRE LEYLEEVPPG NVLGIGGLQD FVLKSATLCS LPSCPPFIPL NFEATPIVRV AVEPKHPSEM PQLVKGMKLL NQADPCVQIL IQETGEHVLV TAGEVHLQRC LDDLKERFAK IHISVSEPII PFRETITKPP KVDMVNEEIG KQQKVAVIHQ MKEDQSKIPE GIQVDSDGLI TITTPNKLAT LSVRAMPLPE EVTQILEENS DLIRSMEQLT SSLNEGENTH MIHQKTQEKI WEFKGKLEQH LTGRRWRNIV DQIWSFGPRK CGPNILVNKS EDFQNSVWTG PADKASKEAS RYRDLGNSIV SGFQLATLSG PMCEEPLMGV CFVLEKWDLS KFEEQGASDL AKEGQEENET CSGGNENQEL QDGCSEAFEK RTSQKGESPL TDCYGPFSGQ LIATMKEACR YALQVKPQRL MAAMYTCDIM ATGDVLGRVY AVLSKREGRV LQEEMKEGTD MFIIKAVLPV AESFGFADEI RKRTSGLASP QLVFSHWEII PSDPFWVPTT EEEYLHFGEK ADSENQARKY MNAVRKRKGL YVEEKIVEHA EKQRTLSKNK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EFNA1 蛋白
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Ephrin A1 (EFNA1) (Active) protein (His tag,Fc Tag)
EFNA1 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE Active
Ephrin A1 (EFNA1) (AA 1-205) protein (GST tag)EFNA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ephrin A1 (EFNA1) (AA 21-130) protein (GST tag)EFNA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ephrin A1 (EFNA1) (AA 1-1127) protein (Strep Tag)Crystallography grade EFNA1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ephrin A1 (EFNA1) (AA 18-182) protein (His tag)Crystallography grade EFNA1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Ephrin A1 (EFNA1) (AA 18-182), (full length) protein (His tag)EFNA1 宿主: 大鼠 宿主: 酵母菌 Recombinant > 90 % ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Ephrin A1 (EFNA1)
- 别名
- EFL1 (EFNA1 产品)
- 别名
- Xefrin-A1 Protein, b61 Protein, ecklg Protein, efl1 Protein, efna1 Protein, efna1a Protein, elfa-a Protein, epha1 Protein, eplg1 Protein, lerk1 Protein, tnfaip4 Protein, xelf-a' Protein, xelfa Protein, AI325262 Protein, B61 Protein, Efl1 Protein, Epl1 Protein, Eplg1 Protein, Lerk1 Protein, ECKLG Protein, EFL1 Protein, EPLG1 Protein, LERK-1 Protein, LERK1 Protein, TNFAIP4 Protein, efna1b Protein, MGC130661 Protein, ephrin-A1 S homeolog Protein, ephrin-A1 Protein, ephrin A1 Protein, ephrin-A1b Protein, ephrin-A1 L homeolog Protein, efna1.S Protein, efna1 Protein, EFNA1 Protein, efna1b Protein, Efna1 Protein, efna1.L Protein
- 背景
- Elongation factor-like GTPase 1 (Elongation factor Tu GTP-binding domain-containing protein 1) (Elongation factor-like 1) (Protein FAM42A),FUNCTION: Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. {ECO:0000269|PubMed:21536732}.
- 分子量
- 125.4 kDa
- UniProt
- Q7Z2Z2
- 途径
- RTK signaling
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