PRAME Protein (AA 1-509) (Strep Tag)
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- 抗原 See all PRAME 蛋白
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-509
- 宿主
- 人
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资源
- Cell-free protein synthesis (CFPS)
- 标记
- This PRAME protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR ELFPPLFMAA FDGRHSQTLK AMVQAWPFTC LPLGVLMKGQ HLHLETFKAV LDGLDVLLAQ EVRPRRWKLQ VLDLRKNSHQ DFWTVWSGNR ASLYSFPEPE AAQPMTKKRK VDGLSTEAEQ PFIPVEVLVD LFLKEGACDE LFSYLIEKVK RKKNVLRLCC KKLKIFAMPM QDIKMILKMV QLDSIEDLEV TCTWKLPTLA KFSPYLGQMI NLRRLLLSHI HASSYISPEK EEQYIAQFTS QFLSLQCLQA LYVDSLFFLR GRLDQLLRHV MNPLETLSIT NCRLSEGDVM HLSQSPSVSQ LSVLSLSGVM LTDVSPEPLQ ALLERASATL QDLVFDECGI TDDQLLALLP SLSHCSQLTT LSFYGNSISI SALQSLLQHL IGLSNLTHVL YPVPLESYED IHGTLHLERL AYLHARLREL LCELGRPSMV WLSANPCPHC GDRTFYDPEP ILCPCFMPN
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- 纯化方法
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
- 纯度
- approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- 质量等级
- custom-made
- Top Product
- Discover our top product PRAME 蛋白
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想要此Protein的其他选项吗？
!探索我们的目录蛋白和定制蛋白服务选项！Product表达系统ConjugateOriginPrice表达系统 Wheat germConjugate GST tagOrigin HumanPrice 6,623.29 ¥表达系统 Escherichia coli (E. coli)Conjugate His-IF2DI TagOrigin HumanPrice 4,466.57 ¥表达系统 Escherichia coli (E. coli)ConjugateOrigin HumanPrice 1,659.45 ¥表达系统 HEK-293 CellsConjugate DYKDDDDK TagOrigin HumanPrice 4,898.90 ¥表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 5,593.03 ¥表达系统 Escherichia coli (E. coli)Conjugate His tagOrigin HumanPrice 8,124.95 ¥表达系统 Escherichia coli (E. coli)Conjugate His tag,GST tagOrigin HumanPrice 8,124.95 ¥表达系统 HEK-293 CellsConjugate Fc TagOrigin HumanPrice 8,284.58 ¥表达系统 HEK-293 CellsConjugate Myc-DYKDDDDK TagOrigin HumanPrice 12,528.17 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (His tag)
custom-made PRAME 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (GST tag)PRAME 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 321-420) protein (His-IF2DI Tag)PRAME 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Preferentially Expressed Antigen in Melanoma (PRAME) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)PRAME 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. - 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- 别名
- PRAME (PRAME 产品)
- 背景
- Melanoma antigen preferentially expressed in tumors (Opa-interacting protein 4) (OIP-4) (Preferentially expressed antigen of melanoma),FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed:21822215, PubMed:26138980). The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding (PubMed:26138980). In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation (PubMed:21822215). Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis (PubMed:16179254). {ECO:0000269|PubMed:16179254, ECO:0000269|PubMed:21822215, ECO:0000269|PubMed:26138980}.
- 分子量
- 57.9 kDa
- UniProt
- P78395
- 途径
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
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