PRAME 蛋白
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- 抗原 See all PRAME 蛋白
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 序列
- MNPLETLSIT NCRLSEGDVM HLSQSPSVSQ LSVLSLSGVM LTDVSPEPLQ ALLERASATL QDLVFDECGI TDDQLLALLP SLSHCSQLTT LSFYGNSISI
- 产品特性
- Length (AA): 106
- 纯度
- > 98 % by SDS-PAGE. Visualized by silver stain
- Top Product
- Discover our top product PRAME 蛋白
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Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (GST tag)
PRAME 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 321-420) protein (His-IF2DI Tag)PRAME 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (Strep Tag)Crystallography grade PRAME 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509), (full length) protein (His tag)PRAME 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509), (full length) protein (His tag)PRAME 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
Preferentially Expressed Antigen in Melanoma (PRAME) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)PRAME 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Positive control for WB.
- 说明
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Cytokines & Growth Factors
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Human PRAME should be reconstituted in water to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffer solutions or stored at -20 °C
- 缓冲液
- 10 mM Tris, 25 mM NaP, pH 7.4
- 储存条件
- 0 °C
- 储存方法
- The lyophilized human PRAME, though stable at room temperature, is best stored desiccated below 0 °C. Reconstituted human PRAME should be stored in working aliquots at -20 °C.
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- 抗原
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- 别名
- PRAME (PRAME 产品)
- 别名
- PRAME Protein, CT130 Protein, MAPE Protein, OIP-4 Protein, OIP4 Protein, 4930534P07Rik Protein, preferentially expressed antigen in melanoma Protein, PRAME Protein, Prame Protein
- 背景
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PRAME/MAPE/OIP4 is a germinal tissue-specific gene that is also expressed at high levels in haematological malignancies and solid tumors. The physiological functions of PRAME in normal and tumor cells are unknown, although a role in the regulation of retinoic acid signaling has been proposed. Sequence homology and structural predictions suggest that PRAME is related to the Leucine-rich repeat (LRR) family of proteins, which have diverse functions. PRAME, or „preferentially expressed antigen in melanoma”, was originally identified as a gene encoding a HLA-A24 restricted antigenic peptide presented to autologous tumor-specific cytotoxic T lymphocytes derived from a patient with melanoma. PRAME is synonymous with MAPE (melanoma antigen preferentially expressed in tumors) and OIP4 (OPA-interacting protein 4), and its expression profile defines it as a cancer-testis antigen. Cancer-testis antigens (CTAs) are encoded by non-mutated genes expressed at high levels in germinal tissues and tumors, but which are absent from or detected at low levels in other tissues. PRAME may be somewhat different to other cancer-testis antigens in that it shows some expression in normal tissues such as ovary, adrenal, placenta and endometrium. The C-terminus of human PRAME (amino acids 453-509) was also identified to bind Neisseria gonorrhoeae opacity factors, in this case the OPA-P protein. Thus PRAME is also known as OIP4 (OPA interacting protein), although the functional implications of the interaction are unknown.
Synonyms: Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, MAPE, OIP4 - 分子量
- 10.7 kDa
- NCBI登录号
- NM_006115, NP_006106
- UniProt
- P78395
- 途径
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
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