MPZ Protein (AA 30-153) (His tag)
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- 抗原 See all MPZ 蛋白
- MPZ (Myelin Protein Zero (MPZ))
- 蛋白类型
- Recombinant
- 产品特性
- AA 30-153
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This MPZ protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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IVVYTDREVH GAVGSRVTLH CSFWSSEWVS DDISFTWRYQ PEGGRDAISI FHYAKGQPYI DEVGTFKERI QWVGDPRWKD GSIVIHNLDY SDNGTFTCDV KNPPDIVGKT SQVTLYVFEK VPTR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MPZ Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product MPZ 蛋白
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Myelin Protein Zero (MPZ) (AA 1-258) protein (GST tag)
MPZ 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Myelin Protein Zero (MPZ) (AA 30-153) protein (His tag)Crystallography grade MPZ 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Myelin Protein Zero (MPZ) (AA 30-248) protein (rho-1D4 tag)Crystallography grade MPZ 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Myelin Protein Zero (MPZ) (AA 30-248) protein (rho-1D4 tag)Crystallography grade MPZ 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Myelin Protein Zero (MPZ) (AA 30-153) protein (His tag)MPZ 宿主: 人 宿主: 酵母菌 Recombinant > 90 % ELISA
Myelin Protein Zero (MPZ) (AA 30-156), (partial) protein (His tag)MPZ 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
Myelin Protein Zero (MPZ) protein (Myc-DYKDDDDK Tag)MPZ 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- MPZ (Myelin Protein Zero (MPZ))
- 别名
- MPZ (MPZ 产品)
- 别名
- CHM Protein, CMT1 Protein, CMT1B Protein, CMT2I Protein, CMT2J Protein, CMT4E Protein, CMTDI3 Protein, CMTDID Protein, DSS Protein, HMSNIB Protein, MPP Protein, P0 Protein, Mpp Protein, P-zero Protein, p0 Protein, sc:d0186 Protein, wu:fc04b11 Protein, wu:fi30g06 Protein, zgc:103775 Protein, myelin protein zero Protein, myelin protein zero S homeolog Protein, MPZ Protein, Mpz Protein, mpz Protein, mpz.S Protein
- 背景
- Creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae.
- 分子量
- 15.2 kDa Including tag.
- UniProt
- P25189
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