EIF4E Protein (AA 2-217) (His tag)
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- 抗原 See all EIF4E 蛋白
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-217
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This EIF4E protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- 序列
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ATVEPETTPT PNPPTTEEEK TESNQEVANP EHYIKHPLQN RWALWFFKND KSKTWQANLR LISKFDTVED FWALYNHIQL SSNLMPGCDY SLFKDGIEPM WEDEKNKRGG RWLITLNKQQ RRSDLDRFWL ETLLCLIGES FDDYSDDVCG AVVNVRAKGD KIAIWTTECE NREAVTHIGR VYKERLGLPP KIVIGYQSHA DTATKSGSTT KNRFVV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human EIF4E Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product EIF4E 蛋白
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Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-217) protein (GST tag)
EIF4E 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-217) protein (His tag)EIF4E 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 2-217) protein (His tag)Crystallography grade EIF4E 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Eukaryotic Translation Initiation Factor 4E (EIF4E) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)EIF4E 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- 别名
- EIF4E (EIF4E 产品)
- 别名
- AUTS19 Protein, CBP Protein, EIF4E1 Protein, EIF4EL1 Protein, EIF4F Protein, eif4e-1 Protein, zgc:86680 Protein, EG668879 Protein, Eif4e-ps Protein, If4e Protein, eIF-4E Protein, 0260/09 Protein, 0587/11 Protein, 0589/11 Protein, 0919/12 Protein, 1004/13 Protein, 2 Protein, 7238 Protein, CG4035 Protein, CT13384 Protein, CT39424 Protein, CT39426 Protein, D-eIF4E Protein, Dmel\\CG4035 Protein, Eif4E Protein, Eif4e Protein, IF4E Protein, dEif4e Protein, deIF-4E Protein, deIF4E Protein, eIF 4E Protein, eIF-4E1 Protein, eIF-4E2 Protein, eIF-4EII Protein, eIF-4e Protein, eIF4E Protein, eIF4E-1 Protein, eIF4E-1/2 Protein, eIF4E-2 Protein, eIF4E1 Protein, eIF4EI Protein, eIF4e Protein, eif-4E Protein, eif-4e Protein, eif4e Protein, l(3)07238 Protein, l(3)67Af Protein, l(3)S025007 Protein, l(3)S026009 Protein, l(3)S058711 Protein, l(3)S091912 Protein, cbp Protein, eif4f Protein, eif4e1 Protein, eif4el1 Protein, EIF-4E Protein, zgc:101581 Protein, eukaryotic translation initiation factor 4E Protein, eukaryotic translation initiation factor 4ea Protein, Eukaryotic initiation factor 4E Protein, eucaryotic initiation factor 6 Protein, eukaryotic translation initiation factor 4E L homeolog Protein, eukaryotic translation initiation factor 4E family member 1B S homeolog Protein, translation initiation factor eIF4E Protein, eukaryotic translation initiation factor 4eb Protein, EIF4E Protein, eif4ea Protein, Eif4e Protein, eIF-4E Protein, eif6 Protein, eif4e Protein, eif4e.L Protein, eif4e1b.S Protein, LOC100533326 Protein, eif4eb Protein
- 背景
- Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. {ECO:0000269|PubMed:16271312, ECO:0000269|PubMed:22578813}.
- 分子量
- 25.9 kDa Including tag.
- UniProt
- P06730
- 途径
- BCR Signaling
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