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EIF4E 蛋白

This Recombinant EIF4E protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN7318456
发货至: 中国
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Quick Overview for EIF4E 蛋白 (ABIN7318456)

抗原

See all EIF4E 蛋白
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

蛋白类型

Recombinant

宿主

  • 12
  • 2
  • 1
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  • 1
  • 1
  • 1
人

资源

  • 11
  • 5
  • 1
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  • 1
大肠杆菌(E. Coli)

纯度

> 95 % as determined by reducing SDS-PAGE.
  • 原理

    Recombinant Human EIF4E Protein

    序列

    Met 1-Val217

    产品特性

    Recombinant Human Eukaryotic Translation Initiation Factor 4E is produced by our E.coli expression system and the target gene encoding Met1-Val217 is expressed.

    过滤

    0.2 μm filtered

    内毒素水平

    < 1.0 EU per μg of the protein as determined by the LAL method.

    Biological Activity Comment

    Not validated for activity
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    缓冲液

    Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
    Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

    有效期

    12 months
  • 抗原

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    别名

    EIF4E

    背景

    EIF4E,EIF4EL1,EIF4F,Eukaryotic translation initiation factor 4E,eIF-4E,eIF-4F 25 kDa subunit,mRNA cap-binding protein,Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

    分子量

    Calculated MW: 25.1 kDa

    Observed MW: 25 kDa

    基因ID

    1977

    UniProt

    P06730

    途径

    BCR Signaling
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