UBE2G2 Protein (AA 2-165) (His tag)
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- 抗原 See all UBE2G2 蛋白
- UBE2G2 (Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-165
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宿主
- 人
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资源
- Insect Cells
- 标记
- This UBE2G2 protein is labelled with His tag.
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- 序列
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AGTALKRLMA EYKQLTLNPP EGIVAGPMNE ENFFEWEALI MGPEDTCFEF GVFPAILSFP LDYPLSPPKM RFTCEMFHPN IYPDGRVCIS ILHAPGDDPM GYESSAERWS PVQSVEKILL SVVSMLAEPN DESGANVDAS KMWRDDREQF YKIAKQIVQK SLGL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human UBE2G2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product UBE2G2 蛋白
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Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) protein
UBE2G2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) (AA 1-165) protein (His tag)UBE2G2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) (AA 1-165) protein (GST tag)UBE2G2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) (AA 1-87) protein (GST tag)UBE2G2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) (Transcript Variant 1) protein (His tag)UBE2G2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2G2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- UBE2G2 (Ubiquitin-Conjugating Enzyme E2G 2 (UBE2G2))
- 别名
- UBE2G2 (UBE2G2 产品)
- 别名
- UBC7 Protein, 1110003O05Rik Protein, D10Xrf369 Protein, Ubc7p Protein, wu:fu51c05 Protein, wu:fu56g11 Protein, zgc:112077 Protein, ubc7 Protein, ube2g1 Protein, ube2g2 Protein, wu:fi33c04 Protein, zgc:55974 Protein, ubiquitin conjugating enzyme E2 G2 Protein, ubiquitin-conjugating enzyme E2G 2 Protein, ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast) Protein, ubiquitin-conjugating enzyme E2 G2 Protein, Ubiquitin-conjugating enzyme E2 G2 Protein, ubiquitin conjugating enzyme E2 G2 L homeolog Protein, ubiquitin-conjugating enzyme E2G 1a (UBC7 homolog, yeast) Protein, UBE2G2 Protein, Ube2g2 Protein, ube2g2 Protein, EDI_023910 Protein, CpipJ_CPIJ018658 Protein, Bm1_47630 Protein, ube2g2.L Protein, ube2g1a Protein
- 背景
- Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD). {ECO:0000269|PubMed:20061386, ECO:0000269|PubMed:22607976}.
- 分子量
- 19.4 kDa Including tag.
- UniProt
- P60604
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