TXN Protein (AA 2-105) (His tag)
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- 抗原 See all TXN 蛋白
- TXN (Thioredoxin (TXN))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-105
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宿主
- 人
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资源
- Insect Cells
- 标记
- This TXN protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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VKQIESKTAF QEALDAAGDK LVVVDFSATW CGPCKMIKPF FHSLSEKYSN VIFLEVDVDD CQDVASECEV KCMPTFQFFK KGQKVGEFSG ANKEKLEATI NELV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human TXN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product TXN 蛋白
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Thioredoxin (TXN) protein
TXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Thioredoxin (TXN) (AA 1-105) (Active) proteinTXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Thioredoxin (TXN) (AA 2-109) protein (His tag)TXN 宿主: E. coli 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS-PAGE SDS
Thioredoxin (TXN) (AA 1-105) protein (GST tag)TXN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Thioredoxin (TXN) (AA 1-105) (Active) protein (His tag)TXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Thioredoxin (TXN) (Active) proteinTXN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % as determined by reducing SDS-PAGE. Active
Thioredoxin (TXN) (AA 2-105) protein (His tag)Crystallography grade TXN 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- TXN (Thioredoxin (TXN))
- 别名
- TXN (TXN 产品)
- 别名
- TRDX Protein, TRX Protein, TRX1 Protein, ADF Protein, AW550880 Protein, Trx1 Protein, Txn Protein, trx Protein, TXN Protein, SCM1.18 Protein, trxA3 Protein, thioredoxin Protein, TR-1 Protein, Trx-1 Protein, thioredoxin Protein, thioredoxin 1 Protein, thioredoxin L homeolog Protein, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Protein, thioredoxin pseudogene Protein, cytosolic thioredoxin Trx1 Protein, TXN Protein, Txn1 Protein, txn.L Protein, txn Protein, trx Protein, SCO0885 Protein, PHG25ORF099c Protein, LOC100349046 Protein, Txn Protein, trx1 Protein
- 背景
- Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity., ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
- 分子量
- 12.6 kDa Including tag.
- UniProt
- P10599
- 途径
- Carbohydrate Homeostasis, Cell RedoxHomeostasis
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