TICAM2 Protein (AA 2-235) (His tag)
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- 抗原 See all TICAM2 蛋白
- TICAM2 (Toll-Like Receptor Adaptor Molecule 2 (TICAM2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-235
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This TICAM2 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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GIGKSKINSC PLSLSWGKRH SVDTSPGYHE SDSKKSEDLS LCNVAEHSNT TEGPTGKQEG AQSVEEMFEE EAEEEVFLKF VILHAEDDTD EALRVQNLLQ DDFGIKPGII FAEMPCGRQH LQNLDDAVNG SAWTILLLTE NFLRDTWCNF QFYTSLMNSV NRQHKYNSVI PMRPLNNPLP RERTPFALQT INALEEESRG FPTQVERIFQ ESVYKTQQTI WKETRNMVQR QFIA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human TICAM2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product TICAM2 蛋白
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Toll-Like Receptor Adaptor Molecule 2 (TICAM2) (AA 1-235) protein (His tag)
TICAM2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Toll-Like Receptor Adaptor Molecule 2 (TICAM2) (AA 1-235) protein (GST tag)TICAM2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Toll-Like Receptor Adaptor Molecule 2 (TICAM2) (AA 2-232) protein (His tag)Crystallography grade TICAM2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Toll-Like Receptor Adaptor Molecule 2 (TICAM2) protein (Myc-DYKDDDDK Tag)TICAM2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- TICAM2 (Toll-Like Receptor Adaptor Molecule 2 (TICAM2))
- 别名
- TICAM2 (TICAM2 产品)
- 别名
- MyD88-4 Protein, TICAM-2 Protein, TIRAP3 Protein, TIRP Protein, TRAM Protein, B430113A10 Protein, Tirp Protein, Trif Protein, toll like receptor adaptor molecule 2 Protein, toll-like receptor adaptor molecule 2 Protein, TICAM2 Protein, Ticam2 Protein
- 背景
- Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1, signaling is proposed to occur in early endosomes after endocytosis of TLR4. May also be involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB, however, reports are controversial. Involved in IL-18 signaling and is proposed to function as a sorting adaptor for MYD88 in IL-18 signaling during adaptive immune response., Isoform 2: Proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1 thereby disrupting the association of isoform 1 with TICAM1. May be involved in TLR4 degradation in late endosomes.
- 分子量
- 27.7 kDa Including tag.
- UniProt
- Q86XR7
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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