VEGFB
(Vascular Endothelial Growth Factor B (VEGFB))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌(E. Coli)
应用范围
Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human VEGF-B protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
内毒素水平
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) in the presence of human VEGF 165. The expected ED50 for this effect is 1.0-2.0ug/mL.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
缓冲液
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
注意事项
Resuspend the protein in the desired concentration in proper buffer
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
抗原
VEGFB
(Vascular Endothelial Growth Factor B (VEGFB))
VEGFL Protein, VRF Protein, VEGF-B Protein, Vrf Protein, vascular endothelial growth factor B Protein, VEGFB Protein, Vegfb Protein
背景
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. [UniProtKB/Swiss-Prot Function]