TIMP2 蛋白
-
- 抗原 See all TIMP2 蛋白
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 应用范围
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
-
- Recombinant human TIMP2 protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- TIMP-2 activity was measured by its ability to inhibit human MMP-1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP-2 concentration of approximately 0.5 ug/mL, when using an MMP-1 concentration of 1.6ug/mL.
- Top Product
- Discover our top product TIMP2 蛋白
-
Metalloproteinase Inhibitor 2 (TIMP2) protein (His tag)
TIMP2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (His tag)TIMP2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Metalloproteinase Inhibitor 2 (TIMP2) protein (Fc Tag)TIMP2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (GST tag)TIMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (His tag)Crystallography grade TIMP2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Metalloproteinase Inhibitor 2 (TIMP2) (AA 30-220), (partial) protein (GST tag)TIMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
-
- 应用备注
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
-
- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- 抗原
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
- 别名
- Timp2 (TIMP2 产品)
- 别名
- CSC-21K Protein, DDC8 Protein, timp2l Protein, zgc:65967 Protein, zgc:85799 Protein, D11Bwg1104e Protein, Timp-2 Protein, TIMP-2 Protein, etID32613.12 Protein, fa96h09 Protein, timp2 Protein, wu:fa96h09 Protein, zgc:136726 Protein, TIMP metallopeptidase inhibitor 2 Protein, TIMP metallopeptidase inhibitor 2b Protein, tissue inhibitor of metalloproteinase 2 Protein, TIMP metallopeptidase inhibitor 2a Protein, TIMP metallopeptidase inhibitor 2 L homeolog Protein, TIMP2 Protein, timp2b Protein, Timp2 Protein, timp2a Protein, timp2.L Protein
- 背景
- This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.
- 分子量
- 21.8 kDa
- NCBI登录号
- NP_003246
- 途径
- cAMP Metabolic Process
-
