TIMP2 Protein (His tag)
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- 抗原 See all TIMP2 蛋白
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This TIMP2 protein is labelled with His tag.
- 序列
- Cys27-Pro220
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Human TIMP-2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. See testing image for detail.
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Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (His tag)
TIMP2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (GST tag)TIMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Metalloproteinase Inhibitor 2 (TIMP2) protein (Fc Tag)TIMP2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Metalloproteinase Inhibitor 2 (TIMP2) (AA 30-220), (partial) protein (GST tag)TIMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
- 别名
- TIMP-2 (TIMP2 产品)
- 别名
- CSC-21K Protein, DDC8 Protein, timp2l Protein, zgc:65967 Protein, zgc:85799 Protein, D11Bwg1104e Protein, Timp-2 Protein, TIMP-2 Protein, etID32613.12 Protein, fa96h09 Protein, timp2 Protein, wu:fa96h09 Protein, zgc:136726 Protein, TIMP metallopeptidase inhibitor 2 Protein, TIMP metallopeptidase inhibitor 2b Protein, tissue inhibitor of metalloproteinase 2 Protein, TIMP metallopeptidase inhibitor 2a Protein, TIMP metallopeptidase inhibitor 2 L homeolog Protein, TIMP2 Protein, timp2b Protein, Timp2 Protein, timp2a Protein, timp2.L Protein
- 背景
- CSC-21K, TIMP2, DDC8, TIMP-2,Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs).TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.
- 分子量
- 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Tris-Bis PAGE result.
- UniProt
- P16035
- 途径
- cAMP Metabolic Process
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