Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
特异性
Optimal preservation of protein structure, post-translational modifications and functions.
产品特性
Recombinant human MMP-3 protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
纯度
> 95 % as determined by SDS-PAGE and Coomassie blue staining
内毒素水平
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
MMP-3 activity was measured by its ability to cleave a chromogenic peptide MMP-3 substrate at room temperature. At a MMP-3 concentration of 2.5 ug/mL, 50% cleavage was achieved at an incubation time of approximately 75 minutes.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
限制
仅限研究用
缓冲液
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
注意事项
Resuspend the protein in the desired concentration in proper buffer
储存条件
-80 °C
储存方法
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. [UniProtKB/Swiss-Prot Function]