ASK1 Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all ASK1 (MAP3K5) 蛋白
- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ASK1 protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Standard (STD)
- 产品特性
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- Recombinant human MEKK5 / ASK1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product MAP3K5 蛋白
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Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1180-1374) protein (His tag)
MAP3K5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) protein (GST tag)MAP3K5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1-1380) protein (Strep Tag)Crystallography grade MAP3K5 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1-1374) protein (Strep Tag)Crystallography grade MAP3K5 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) proteinMAP3K5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
- 别名
- Mekk5,ask1 (MAP3K5 产品)
- 别名
- ASK1 Protein, MAPKKK5 Protein, MEKK5 Protein, mitogen-activated protein kinase kinase kinase 5 Protein, MUD21.11 Protein, MUD21_11 Protein, 7420452D20Rik Protein, ASK Protein, Mekk5 Protein, Ask1 Protein, RGD1306565 Protein, mitogen-activated protein kinase kinase kinase 5 Protein, MAP3K5 Protein, MAPKKK5 Protein, Map3k5 Protein, map3k5 Protein
- 背景
- Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells MAPKKK5 does not activate MAPK/ERK.
- 分子量
- 154.4 kDa
- NCBI登录号
- NP_005914
- 途径
- MAPK Pathway, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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