Liver Arginase Protein (Myc-DYKDDDDK Tag)
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- 抗原 See all Liver Arginase (ARG1) 蛋白
- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Liver Arginase protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human Arginase-1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- Arginase activity verified in a biochemical assay: Arginase 1 (ARG1, ) activity was measured in a colorimetric biochemical assay. Arginase 1 catalyzes the conversion of arginine to ornithine and urea. After incubation of the protein in a solution containing L-arginine, the reaction is stopped, and the urea concentration is measured by a chemical reaction that produces a colored product that absorbs at 430 nm. By measuring the absorbance at 430 nm and comparing to a standard, the specific activity of this preparation of ARG1 was calculated to be approximately 10U/mg. Unit definition: 1 unit of ARG1 converts 1 μmole of L-arginine to ornithine and urea per minute at pH 9.5 and 37°C.
- Top Product
- Discover our top product ARG1 蛋白
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Arginase, Liver (ARG1) (AA 1-322) protein (GST tag)
ARG1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Arginase, Liver (ARG1) protein (His tag)ARG1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by reducing SDS-PAGE.
Arginase, Liver (ARG1) protein (MYC tag,His tag)ARG1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Arginase, Liver (ARG1) (AA 1-323) protein (Strep Tag)Crystallography grade ARG1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- Liver Arginase (ARG1) (Arginase, Liver (ARG1))
- 别名
- Arginase-1 (ARG1 产品)
- 别名
- SI:zC146F4.4 (novel protein with NUDIX domain) Protein, si:ch211-146f4.3 Protein, argi1 Protein, AI Protein, AI256583 Protein, Arg-1 Protein, PGIF Protein, arginase 1 Protein, arginase Protein, Arginase-1 Protein, arginase, liver Protein, L-arginase Protein, arg1 Protein, PGTG_16455 Protein, argi1 Protein, ARG1 Protein, Arg1 Protein
- 背景
- Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
- 分子量
- 34.6 kDa
- NCBI登录号
- NP_000036
- 途径
- Cellular Response to Molecule of Bacterial Origin
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