BRD4 Protein (AA 333-460) (His tag,DYKDDDDK Tag)
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- 抗原 See all BRD4 蛋白
- BRD4 (Bromodomain Containing 4 (BRD4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 333-460
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This BRD4 protein is labelled with His tag,DYKDDDDK Tag.
- 应用范围
- Binding Studies (Bind), Screening Assay (ScA)
- 产品特性
- The peptide corresponding to amino acids 333-460 that contains the bromodomain sequences of BRD4 (accession number NP_490597.1) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 20.9 kDa.
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Bromodomain Containing 4 (BRD4) (AA 49-170) protein (His tag)
BRD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Bromodomain Containing 4 (BRD4) proteinBRD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Bromodomain Containing 4 (BRD4) (AA 1-1362) protein (Strep Tag)Crystallography grade BRD4 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Bromodomain Containing 4 (BRD4) (AA 1-1400) protein (Strep Tag)Crystallography grade BRD4 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Bromodomain Containing 4 (BRD4) (AA 44-168) protein (His tag,DYKDDDDK Tag)BRD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Bind, ScA
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- 应用备注
- Recombinant BRD4 (333-460) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
- 限制
- 仅限研究用
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- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -80 °C
- 储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- 抗原
- BRD4 (Bromodomain Containing 4 (BRD4))
- 别名
- BRD4 (BRD4 产品)
- 别名
- brd4-b Protein, BRD4 Protein, fb18g03 Protein, wu:fb18g03 Protein, CAP Protein, HUNK1 Protein, HUNKI Protein, MCAP Protein, Brd5 Protein, WI-11513 Protein, bromodomain containing 4 L homeolog Protein, bromodomain containing 4 Protein, bromodomain-containing protein 4 Protein, brd4.L Protein, BRD4 Protein, brd4 Protein, LOC100409351 Protein, Brd4 Protein
- 背景
- Bromodomain-containing protein 4 (BRD4) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD4 has been identified recently as a therapeutic target in many cancers, including acute myeloid leukemia, multiple myeloma, Burkitt's lymphoma, NUT midline carcinoma, colon cancer, and breast cancer. BRD4 regulates the transcription of oncogenes, HIV, and human papilloma virus (HPV). It has been shown to bind and phosphorylate RNA pol II, which implicates its involvement in the regulation of eukaryotic transcription.
- 分子量
- 20.9 kDa
- 途径
- Chromatin Binding, SARS-CoV-2 Protein Interactome
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