-
- 抗原 See all KDM5A 蛋白
- KDM5A (Lysine (K)-Specific Demethylase 5A (KDM5A))
- 蛋白类型
- Recombinant
- 宿主
- 人
-
资源
- Baculovirus
- 标记
- This KDM5A protein is labelled with DYKDDDDK Tag.
- 应用范围
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- 产品特性
- Recombinant JARID1A / KDM5A (accession number NP_001036068.1) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 196.3 kDa.
-
Lysine (K)-Specific Demethylase 5A (KDM5A) (AA 191-290) protein (GST tag)
KDM5A 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Lysine (K)-Specific Demethylase 5A (KDM5A) (AA 437-603) protein (His tag)KDM5A 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
Lysine (K)-Specific Demethylase 5A (KDM5A) (AA 437-603) protein (His-SUMO Tag)KDM5A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Lysine (K)-Specific Demethylase 5A (KDM5A) protein (DYKDDDDK Tag)KDM5A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- Recombinant JARID1A / KDM5A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Catalytic rate: >60 turnovers/ enzyme molecule/ hour. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JARID1A / KDM5A protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- 限制
- 仅限研究用
-
- 注意事项
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -80 °C
- 储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
-
- 抗原
- KDM5A (Lysine (K)-Specific Demethylase 5A (KDM5A))
- 别名
- JARID1A / KDM5A (KDM5A 产品)
- 别名
- Jarid1a Protein, JARID1A Protein, RBBP-2 Protein, RBBP2 Protein, RBP2 Protein, AA409370 Protein, C76986 Protein, Rbbp2 Protein, lysine demethylase 5A Protein, lysine (K)-specific demethylase 5A Protein, Kdm5a Protein, KDM5A Protein
- 背景
- Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis.
- 分子量
- 196.3 kDa
- 途径
- Chromatin Binding, Warburg Effect
-
You are here:
