NF-kB p65 Protein (full length)
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Quick Overview for NF-kB p65 Protein (full length) (ABIN2669407)
抗原
See all NF-kB p65 (NFkBP65) 蛋白蛋白类型
宿主
资源
应用范围
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产品特性
- full length
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原理
- Recombinant NFkB p65 protein
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产品特性
- Recombinant NFkB p65 protein was expressed from a full-length cDNA clone in E. coli and has a 14 amino acid truncation at the C-term. This clone had five point mutations compared to the p65 sequence listed under accession no. AAA36408: L159V, P180S, F309S, A439V and V462M. The protein was purified by affinity chromatography, followed by gel filtration. NFkB p65 is a subunit of the NFkB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. NFkB p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains, they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFkB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IkB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IkB proteins. This releases NFkB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes. Recombinant NFkB p65 is suitable for Western blotting and TransAM assays.
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纯化方法
- purified by affinity chromatography, followed by gel filtration.
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Nuclear Factor-kB p65 (NFkBP65) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)
NFkBP65 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
Nuclear Factor-kB p65 (NFkBP65) (AA 1-220) protein (GST tag)NFkBP65 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nuclear Factor-kB p65 (NFkBP65) (AA 432-505) protein (GST tag)NFkBP65 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nuclear Factor-kB p65 (NFkBP65) (AA 1-220) protein (His-IF2DI Tag)NFkBP65 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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应用备注
- Recombinant NFkB p65 is suitable for Western blotting and TransAM assays. 100 ng is sufficient for most protein-protein interaction studies. The standard curve for TransAM NFkB p65 was generated using the range of 40-0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided. For larger package sizes of Recombinant NFkB p65, please refer to Cat. No. 81086 / 81786.
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限制
- 仅限研究用
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浓度
- 100 ng/μL
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缓冲液
- 5 μg of Recombinant NFkB p65 protein 1X Protein Dilution Buffer (20 mM Tris-HCl, 0.2 M NaCl, 2 mM MgCl2 and 10 % glycerol) and 1 M DTT. Protein is supplied at 100 ng/ul in Protein Dilution Buffer.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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别名
- NFkappaB p65
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背景
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Protein Background: NFkB p65 is a subunit of the NFkB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. NFkB p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains, they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFkB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IkB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IkB proteins. This releases NFkB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.
Short Description: Recombinant NFkB p65 protein was expressed from a full-length cDNA clone in E. coli and has a 14 amino acid truncation at the C-term. This clone had five point mutations compared to the p65 sequence listed under accession no. AAA36408: L159V, P180S, F309S, A439V and V462M. The protein was purified by affinity chromatography, followed by gel filtration. The protein contains a His-Tag.
Aliases: RELA, p65, NFKB3
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途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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