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TNF alpha Protein (AA 80-235)

Name: TNF alpha Protein (AA 80-235)
SKU: ABIN2667596
Availability: OutOfStock

This Recombinant TNF alpha protein is expressed in 大肠杆菌（E. Coli）.

产品编号 ABIN2667596

发货至: 中国

Quick Overview for TNF alpha Protein (AA 80-235) (ABIN2667596)

抗原

See all TNF alpha 蛋白

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

蛋白类型

Recombinant

宿主

小鼠

资源

大肠杆菌（E. Coli）

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF)

纯度

Purity is >98 % as determined by SDS PAGE , Coomassie stained with Invitrogen SimplyBlue SafeStain.

Discover our top product TNF alpha 蛋白

产品特性

AA 80-235

过滤

0.22 μm filtered
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Product

表达系统

Conjugate

Origin

价格从

TNF alpha Protein (AA 1-235) (His tag)

表达系统 HEK-293 Cells

Conjugate His tag

Origin Mouse

价格从 136,947.45 ¥

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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)
custom-made TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)
custom-made TNF alpha 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein
HPLC verified TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 兔宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active

应用备注

Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Lyophilized

溶解方式

Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

缓冲液

Lyophilized in 0.22μm filtered PBS, pH 7.2, containing 1 % BSA.

注意事项

Use a manual defrost freezer and avoid repeated freeze thaw cycles.

储存条件

4 °C

储存方法

Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

有效期

12 months
抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF-alpha

背景

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes, stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

分子量

The 156 amino acid protein has a predicted molecular mass of 17,257 Da. The DTT-reduced and non-reduced protein migrates at approximately 16 kDaa by SDS-PAGE. The N-terminal amino acid is Leu.

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome