NGFB Protein (AA 122-239)
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Quick Overview for NGFB Protein (AA 122-239) (ABIN2667381)
抗原
See all NGFB 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 122-239
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内毒素水平
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Less than 0.1 ng per μg of protein.
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Nerve Growth Factor beta (NGFB) (Active) protein
NGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Biological activity: Assay 1: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg. Assay 2: Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.
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缓冲液
- Lyophilized, carrier-free.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- NGFB (Nerve Growth Factor beta (NGFB))
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别名
- beta-NGF
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背景
- β-NGF is a pluripotent mediator and it exists in many tissues. Secreted β-NGF can bind to tryosine kinase A receptor with higher affinity and to p75 (NTR) with low affinity. β-NGF is well known as an endogenous neurotrophic factor involved in the embryonal development, survival, and regeneration of mammalian sympathetic and sensory neurons. In adults, β-NGF is also involved in the survival and regeneration of neurons in the central nervous system. Involvement of β-NGF in the immune system has also been reported. In vitro cultured B lymphocyte can constitutively secret β-NGF, which can act as an autocrine growth and differentiation factor for B cells. β-NGF can also downregulate the production of IFN-γ by T cells. Recombinant β-NGF has been used in many clinical trials to treat different disease including Alzheimer's disease, diabetic polyneuropathy, and HIV-associated peripheral neuropathy.
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分子量
- The 118 amino acid recombinant protein has a predicted molecular mass of approximately 13.5 kDa. This protein is a disulfide-linked homodimer and the predicted molecular mass is 27 kDa. The predicted N-terminal amino acid is Ser.
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途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
抗原
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