SIGLEC5 Protein (AA 17-434, C-Term) (Fc Tag)
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- 抗原 See all SIGLEC5 蛋白
- SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 17-434, C-Term
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宿主
- 人
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资源
- CHO Cells
- 标记
- This SIGLEC5 protein is labelled with Fc Tag.
- 应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 1.0 EU per μg of protein as determined by the LAL method.
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Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-434) protein (His-Avi Tag)
SIGLEC5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-434) protein (Fc Tag)SIGLEC5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-439) protein (His tag)SIGLEC5 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-434) protein (His-Avi Tag,Biotin)SIGLEC5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 465-549) protein (GST tag)SIGLEC5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-434) protein (Fc Tag,Biotin)SIGLEC5 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) protein (His tag)SIGLEC5 宿主: Cynomolgus 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-441) protein (His tag)Crystallography grade SIGLEC5 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-439) protein (His tag)Crystallography grade SIGLEC5 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 461-569) protein (His tag)Crystallography grade SIGLEC5 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Recombinant human Siglec 5 is able to agglutinate human red blood cells with an ED50 of 0.625 - 2.5 μg/mL.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA. After dilution, the cytokine can be stored at 4 °C for one month or from -20 °C to -70 °C for up to three months.
- 浓度
- 100 μg/mL
- 缓冲液
- 0.22 μm filtered protein solution is in 20 mM Tris, 150 mM NaCl, and pH 7.4.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
- 别名
- Siglec 5 (SIGLEC5 产品)
- 别名
- CD170 Protein, CD33L2 Protein, OB-BP2 Protein, OBBP2 Protein, SIGLEC-5 Protein, sialic acid binding Ig like lectin 5 Protein, sialic acid-binding Ig-like lectin 5 Protein, sialic acid binding Ig-like lectin 5 Protein, SIGLEC5 Protein, LOC100597560 Protein, Siglec5 Protein
- 背景
- Siglec proteins (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region which contains a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. In the cytosolic domain, most siglecs contain a combination of tyrosine motifs that include immunoreceptor tyrosine-based inhibitory motifs (ITIMs) along with ITIM-like, Grb2-binding, and Fyn kinase sites. Siglecs are widely expressed on hematopoietic cells, often in a cell type specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell surface glycoproteins and glycolipids. Studies suggest that Siglecs may participate in cell-to-cell interactions or can act as receptors during the entry of viral or bacterial pathogens. In addition, the presence of ITIMs indicate that these molecules play a role in the suppression of immunoreceptor signaling. Siglecs can be classified into two subgroups with Siglec 1, 2, and 4 as one group and a Siglec 3/CD33-related subgroup (Siglec 3 and Siglec 5 - 14) as the second. Siglec 5, a human CD33-related Siglec, selectively regulates early recruitment of neutrophils in the lung during lipopolysaccharide-induced acute lung inflammation.
- 分子量
- This 652 amino acid recombinant protein has a predicted molecular mass of approximately 72.7 kDa. The protein migrates at approximately 120 kDa in DTT-reduced conditions and approximately 250 kDa in non-reduced conditions by SDS-PAGE. The predicted N-term
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