CD33 Protein (CD33) (AA 18-259, C-Term) (Fc Tag)
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- 抗原 See all CD33 蛋白
- CD33
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 18-259, C-Term
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD33 protein is labelled with Fc Tag.
- 应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.1 EU per μg of protein as determined by the LAL method.
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- Discover our top product CD33 蛋白
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CD33 (CD33) (AA 18-259) protein (His-Avi Tag)
CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD33 (CD33) (AA 18-259) protein (Fc Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD33 (CD33) (AA 18-259) protein (His-Avi Tag,Biotin)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
CD33 (CD33) (AA 18-240) protein (His tag)CD33 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD33 (CD33) (AA 17-248) protein (His tag)CD33 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD33 (CD33) protein (Fc-His Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD33 (CD33) (AA 1-364) protein (GST tag)CD33 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
CD33 (CD33) (AA 18-259) protein (FITC,His-Avi Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Recombinant human Siglec 3 is able to agglutinate human red blood cells with an ED50 = 0.5 - 4.0 μg/mL.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA. After dilution, the cytokine can be stored at 4 °C for one month or from -20 °C to -70 °C for up to three months.
- 浓度
- 100 μg/mL
- 缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- CD33
- 别名
- Siglec 3 (CD33 产品)
- 别名
- SIGLEC-3 Protein, SIGLEC3 Protein, p67 Protein, Siglec-3 Protein, gp67 Protein, CD33 molecule Protein, CD33 antigen Protein, CD33 Protein, Cd33 Protein
- 背景
- Siglec proteins (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. In the cytosolic domain, most Siglecs contain a combination of tyrosine motifs, including immunoreceptor tyrosine-based inhibitory motifs (ITIM), along with ITIM-like, Grb2-binding, and Fyn kinase sites. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Studies suggest that Siglecs may participate in cell-cell interactions and act as receptors for the entry of viral or bacterial pathogens. In addition, the presence of ITIM indicates that these molecules play a role in the suppression of immunoreceptor signaling. Siglecs can be classified into two subgroups with Siglec 1, 2, and 4, as one group and a Siglec 3/CD33-related subgroup (Siglec 3 and 5 through 14) as the second. Siglec 3, also known as CD33 and GP67, is mainly expressed on myeloid cells and also on activated NK and T cells. Human Siglec 3 has two tyrosine residues in its cytoplasmic domain. When phosphorylated, these tyrosines could confer an inhibitory function. CD33 polymorphism has been associated with late-onset Alzheimer's disease.
- 分子量
- This 493 amino acid recombinant protein has a predicted molecular mass of approximately 54.9 kDa. The protein migrates approximately at 70 kDa in DTT-reducing conditions and at 150 kDa in non-reducing conditions by SDS-PAGE. The predicted N-terminal amino
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