TGFB3 Protein (AA 301-412)
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- 抗原 See all TGFB3 蛋白
- TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 301-412
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宿主
- 人
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资源
- CHO Cells
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 98 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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- Discover our top product TGFB3 蛋白
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Transforming Growth Factor, beta 3 (TGFB3) (AA 301-412) protein
TGFB3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Transforming Growth Factor, beta 3 (TGFB3) (AA 301-412) protein (AVI tag,Biotin)TGFB3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Transforming Growth Factor, beta 3 (TGFB3) (Active) proteinTGFB3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Transforming Growth Factor, beta 3 (TGFB3) (AA 1-412) protein (GST tag)TGFB3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: TGF-β3 inhibits the proliferation of mouse HT-2 cells induced by IL-4. The ED50 is from 0.10 to 0.4 ng/ml, corresponding to a specific activity of 2.5-10 x 106 units/mg.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HAS or 10 % FBS. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 缓冲液
- 0.22 μm filtered protein solution is in 25 % Acetonitrile, 0.1 % TFA (trifluoroacetic acid).
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
- 别名
- TGF-Beta3 (TGFB3 产品)
- 别名
- TGFB3 Protein, ARVD Protein, TGF-beta3 Protein, Tgfb-3 Protein, TGF-B3 Protein, TGFbeta3 Protein, wu:fc78g09 Protein, zgc:92058 Protein, transforming growth factor beta 3 Protein, transforming growth factor, beta 3 Protein, TGFB3 Protein, Tgfb3 Protein, tgfb3 Protein
- 背景
- Human TGF-β3 precursor consists of two disulfide-linked identical subunits with 412 amino acids each. The carboxy-terminal (112 residues) of TGF-β3 represents the biologically active form of this protein, this portion of TGF-β 3 exhibits 86/112 and 89/112 identical residues compared with TGF-β1 and TGF-β2, respectively. TGF-β3 precursor is cleaved by furin, and localized in the residue 300 of the trans-Golgi network. Furin processes the TGF-β3 precursor at the carboxyl side of the consensus sequence RKKR which precedes the NH2-terminal Ala 301 residue of the mature TGF-β3. The TGF-β3 precursor includes (in the N-terminal portion) the latency-associated peptide (LAP dimer) and (in the C-terminal) the 25 kD portion that constitutes the mature TGF-β3. The LAP dimer and the TGF-β3 mature protein remain non-covalently associated after furin cleavage, and this complex does not bind to the TFG-β receptor. In addition, the TGF-β3 latent complex is joined covalently through LAP to LTBP. The TGF-β1 active form requires dissociation from LAP. Some activators can release TGF-β1 from LAP, such as thrombospondin-1, reactive oxygen species, and the integrins avb6 and avb8, and these activators might release TGF-β3 through similar mechanism. TGF-β3 induces biomechanical remodeling in avian embryonic atrioventricular valves. Also, gene delivery of a mutant TGF-β3 reduces re-epithelialization density and fibroblast/myofibroblast transdifferentiation within the wound area, both mechanisms are indicative of reduced scar tissue formation. In fact, recombinant TGF-β3 has been used in clinical trials as prophylactic treatment of human scars.
- 分子量
- The 112 amino acid recombinant protein has a predicted molecular mass of approximately 12.7 kDa. The DTT-reduced protein migrates at approximately 14 kDa and non-reduced protein migrates at 28-30 kDa by SDS-PAGE. The N-terminal amino acid is Alanine.
- 途径
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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