TGFB3 Protein (AA 301-412)
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北京 101111
Quick Overview for TGFB3 Protein (AA 301-412) (ABIN7275712)
抗原
See all TGFB3 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 301-412
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原理
- Human Mature TGF beta 3 Protein
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序列
- Ala301-Ser412
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产品特性
- Recombinant Human Mature TGF beta 3 Protein is expressed from HEK293 without tag.It contains Ala301-Ser412.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human Mature TGF beta 3, No Tag at 1ug (100ul/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test).,Human Mature TGF beta 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 10-80 pg/ml (QC Test).,Loaded Human TGF-beta RII, hFc Tag on ProA-Biosensor can bind Human Mature TGF beta 3, No Tag with an affinity constant of 0.51 nM as determined in BLI assay (Gator® Prime).
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Transforming Growth Factor, beta 3 (TGFB3) (AA 301-412) (Active) protein (AVI tag,Biotin)
TGFB3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Transforming Growth Factor, beta 3 (TGFB3) (Active) proteinTGFB3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Transforming Growth Factor, beta 3 (TGFB3) (AA 24-412) protein (His tag)TGFB3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
Transforming Growth Factor, beta 3 (TGFB3) (AA 1-412) protein (GST tag)TGFB3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
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别名
- TGF beta 3
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背景
- Transforming growth factor-beta 3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. TGF beta 3 RNA is expressed in a diverse array of tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, and amnion, as well as in central nervous system (CNS) structures such as the meninges, choroid plexus and the olfactory bulbs. Furthermore, in several organ systems, TGF beta 3 transcripts are expressed during periods of active morphogenesis suggesting that the protein may be an important factor for the growth and differentiation of many embryonic tissues.
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分子量
- 12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
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UniProt
- P10600
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途径
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
抗原
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