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TNF alpha Protein (AA 80-235)

Name: TNF alpha Protein (AA 80-235)
SKU: ABIN2666709
Availability: OutOfStock

This Recombinant TNF alpha protein is expressed in 大肠杆菌（E. Coli）.

产品编号 ABIN2666709

发货至: 中国

Quick Overview for TNF alpha Protein (AA 80-235) (ABIN2666709)

抗原

See all TNF alpha 蛋白

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

蛋白类型

Recombinant

生物活性

Active

宿主

大鼠

资源

大肠杆菌（E. Coli）

应用范围

Immunoprecipitation (IP), Flow Cytometry (FACS)

纯度

Purity is > 98 % , as determined by Coomassie stained SDS-PAGE.

Discover our top product TNF alpha 蛋白

产品特性

AA 80-235

过滤

0.22 μm filtered

内毒素水平

Endotoxin level is <0.1 EU/μg (<0.01 ng/μg) protein as determined by the LAL method.
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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)
custom-made TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)
custom-made TNF alpha 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein
HPLC verified TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 兔宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active

应用备注

Optimal working dilution should be determined by the investigator.

说明

Biological activity: The ED50 is 5- 15 pg/ml, corresponding to a specific activity of 2 - 0.66 x 108units/mg, as determined by a dose dependent cytotoxicity assay using L929 cells treated with actinomycin D.

限制

仅限研究用
状态

Liquid

溶解方式

For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

缓冲液

0.22μm filtered protein solution is in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.

注意事项

Avoid repeated freeze/thaw cycles.

储存条件

-20 °C

储存方法

Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF-alpha

背景

TNF-α was originally described as an endotoxin-induced, macrophage-derived factor that promotes hemorrhagic necrosis of solid tumors and the cachexia of chronic infections. TNF-α has also been implicated in a range of inflammatory, infectious, and malignant disorders. At the cellular level, TNF-α modulates a broad spectrum of responses including inflammation, immunoregulation, proliferation, apoptosis, and antiviral activity. In bone, the cytokine inhibits extracellular matrix deposition, stimulates matrix metalloprotease synthesis, and enhances production of osteoclastogenic cytokines such as M-CSF and RANKL. Chronic exposure to TNFa in vivo increases osteoclastogenesis through two distinct mechanisms. TNFa first affects osteoclastogenesis at the osteoclast precursor stage in the bone marrow by priming these cells to differentiate into cFms+/CD11b+/RANK+/- osteoclast progenitors via a RANKL/RANK independent mechanism. These osteoclast precursors then enter the blood and peripheral tissues where they differentiate into mature osteoclasts in the presence of RANKL, and this process is accelerated by TNF. The role of TNF at this later stage of osteoclast differentiation is RANKL/RANK dependent. Importantly, TNF-α promotes bone resorption both in vitro and in vivo by enhancing the proliferation and differentiation of osteoclast precursors.

分子量

The 157 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 17,361 Da. The DTT-reduced protein migrates at approximately 17kDa and the non-reduced protein migrates at approximately 16kDa by SDS-PAGE.

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome