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UBE2D1 Protein (AA 1-147) (GST tag)

UBE2D1 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
产品编号 ABIN2115460
发货至: 中国
  • 抗原 See all UBE2D1 蛋白
    UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))
    蛋白类型
    Recombinant
    产品特性
    AA 1-147
    宿主
    • 9
    • 1
    • 1
    资源
    • 5
    • 2
    • 2
    • 1
    • 1
    大肠杆菌(E. Coli)
    标记
    This UBE2D1 protein is labelled with GST tag.
    原理
    Recombinant Human Ubiquitin-Conjugating Enzyme E2 D1/UBE2D1/UbcH5a (N-GST)
    序列
    MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMALK RIQKELSDLQ RDPPAHCSAG PVGDDLFHWQ ATIMGPPDSA YQGGVFFLTV HFPTDYPFKP PKIAFTTKIY HPNINSNGSI CLDILRSQWS PALTVSKVLL SICSLLCDPN PDDPLVPDIA QIYKSDKEKY NRHAREWTQK YAM
    产品特性
    Recombinant Human Ubiquitin-conjugating enzyme E2 D1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Met147) of Human UBE2D1 fused with a GST tag at the N-terminus.
    纯度
    > 95 % as determined by reducing SDS-PAGE.
    过滤
    0.2 μm filtered
    内毒素水平
    Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
    Top Product
    Discover our top product UBE2D1 蛋白
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    缓冲液
    Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES,150 mM NaCl,2 mM DTT,10 % Glycerin pH 7.5.
    储存液
    Dithiothreitol (DTT)
    注意事项
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    储存条件
    4 °C/-20 °C/-80 °C
    储存方法
    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • 抗原
    UBE2D1 (Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1))
    别名
    Ubiquitin-Conjugating Enzyme E2 D1 (UBE2D1 产品)
    别名
    E2(17)KB1 Protein, SFT Protein, UBC4/5 Protein, UBCH5 Protein, UBCH5A Protein, ube2d1 Protein, wu:fc16h06 Protein, zgc:73096 Protein, e2(17)kb1 Protein, sft Protein, ubc4/5 Protein, ubch5 Protein, ubch5a Protein, ubiquitin conjugating enzyme E2 D1 Protein, ubiquitin-conjugating enzyme E2D 1 Protein, ubiquitin-conjugating enzyme E2D 1b Protein, ubiquitin conjugating enzyme E2 D1 S homeolog Protein, UBE2D1 Protein, Ube2d1 Protein, ube2d1b Protein, ube2d1.S Protein, ube2d1 Protein
    背景
    Ubiquitin-conjugating enzyme E2 D1(UBE2D1)belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
    Synonyms: Ubiquitin-conjugating enzyme E2 D1,Stimulator of Fe transport,SFT,UBC4/5 homolog,UbcH5,Ubiquitin carrier protein D1,Ubiquitin-conjugating enzyme E2(17)KB 1,Ubiquitin-conjugating enzyme E2-17 kDa 1,Ubiquitin-protein ligase D1,SFT, UBC5A, UBCH5, UBCH5A
    分子量
    42.9 kDa
    UniProt
    P51668
    途径
    Activation of Innate immune Response, Toll-Like Receptors Cascades
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