ED50 < 0.5 μg/mL, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of > 2.0x 10^3 units/mg. AA 19-178 (Arg25Lys, Leu65Val), expressed with an N-terminal Met.
IL10
宿主: Pig
宿主: 大肠杆菌(E. Coli)
Recombinant
>95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstituted in ddH2O at 100 μg/mL.
缓冲液
Lyophilized after extensive dialysis against PBS.
储存条件
-80 °C
储存方法
Lyophilized recombinant rat Interleukin-10 (IL-10) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, IL-10 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine produced by a variety of cell lines including T-cells, macrophages and mast cells. IL-10 is classified as a class-2 cytokine, a set of cytokines including IL-19, IL-20, IL-22, IL-24, and IL-26. IL-10 can inhibit the synthesis of pro-inflammatory cytokines such as IFN-gamma, IL-2, IL-3, TNF and GM-CSF. It also stimulates Th2 responses, but suppresses the antigen-presentation capacity of antigen presenting cells.Recombinant rat Interleukin-10 (IL-10)produced in E. coli is a single non-glycosylated polypeptide chain containing 161 amino acids. A fully biologically active molecule, recombinant rat Interleukin-10 (IL-10) has a molecular mass of 18.7 kDa analyzed by reducing SDS-PAGE. Synonyms: Cytokine synthesis inhibitory factor, CSIF, TGIF, IL10A