UBE2V1 Protein (AA 2-147) (His tag)
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- 抗原 See all UBE2V1 蛋白
- UBE2V1 (Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-147
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This UBE2V1 protein is labelled with His tag.
- 原理
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 1/UBE2V1 (C-6His)
- 序列
- AATTGSGVKV PRNFRLLEEL EEGQKGVGDG TVSWGLEDDE DMTLTRWTGM IIGPPRTIYE NRIYSLKIEC GPKYPEAPPF VRFVTKINMN GVNSSNGVVD PRAISVLAKW QNSYSIKVVL QELRRLMMSK ENMKLPQPPE GQCYSNLEHH HHHH
- 产品特性
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 1/UBE2V1 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Asn147) of Human UBE2V1 fused with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product UBE2V1 蛋白
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Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 1-147) protein (His tag)
UBE2V1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 2-170) protein (His tag)UBE2V1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (AA 1-147) protein (GST tag)UBE2V1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)UBE2V1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2V1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 100 mM NaCl, pH 8.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- UBE2V1 (Ubiquitin-Conjugating Enzyme E2 Variant 1 (UBE2V1))
- 别名
- UBE2V1 (UBE2V1 产品)
- 别名
- CIR1 Protein, CROC-1 Protein, CROC1 Protein, UBE2V Protein, UEV-1 Protein, UEV1 Protein, UEV1A Protein, 0610011J09Rik Protein, AI256840 Protein, D7Bwg1382e Protein, CROC-1B Protein, ubiquitin conjugating enzyme E2 V1 Protein, ubiquitin-conjugating enzyme E2 variant 1 Protein, UBE2V1 Protein, ube2v1 Protein, Ube2v1 Protein
- Sub Type
- Fusionprotein
- 背景
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Ubiquitin-Conjugating Enzyme Variant 1a (UBE2V1) is a member of the Ubiquitin-conjugating (E2) enzyme family. The E2 catalytic core domain of UBE2V1 lacks an active site cysteine residue, rendering it catalytically inactive on its own. However, in the cytoplasm UBE2V1 is able to form a catalytically active complex with UBE2N/Ubc13, which mediates the synthesis Lys63-linked Ubiquitin chains and is required for NF-kappa B activation. UBE2V1 is required for UBE2N (Ubc13)/UBE2V1 Complex-dependent Lys63-linked Ubiquitin chain formation. More specifically, UBE2V1 orients the Ubiquitin molecule to favor linkage at Lys63 via a non-covalent interaction with the Ubiquitin molecule. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. UBE2V1 plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6, and TRAF2. It mediates transcriptional activation of target genes. UBE2V1 also controls the progress through the cell cycle and differentiation, the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
Alternative Names: Ubiquitin-Conjugating Enzyme E2 Variant 1, UEV-1, CROC-1, TRAF6-Regulated IKK Activator 1 Beta Uev1A, UBE2V1, CROC1, UBE2V, UEV1, P/OKcl.19 - 分子量
- 17.5 kDa
- UniProt
- Q13404
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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