SMAD4 Protein (AA 1-552) (His tag)
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- 抗原 See all SMAD4 蛋白
- SMAD4 (SMAD Family Member 4 (SMAD4))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-552
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SMAD4 protein is labelled with His tag.
- 原理
- Recombinant Human SMAD Family Member 4/SMAD4/DPC4 (C-6His)
- 序列
- MDNMSITNTP TSNDACLSIV HSLMCHRQGG ESETFAKRAI ESLVKKLKEK KDELDSLITA ITTNGAHPSK CVTIQRTLDG RLQVAGRKGF PHVIYARLWR WPDLHKNELK HVKYCQYAFD LKCDSVCVNP YHYERVVSPG IDLSGLTLQS NAPSSMMVKD EYVHDFEGQP SLSTEGHSIQ TIQHPPSNRA STETYSTPAL LAPSESNATS TANFPNIPVA STSQPASILG GSHSEGLLQI ASGPQPGQQQ NGFTGQPATY HHNSTTTWTG SRTAPYTPNL PHHQNGHLQH HPPMPPHPGH YWPVHNELAF QPPISNHPAP EYWCSIAYFE MDVQVGETFK VPSSCPIVTV DGYVDPSGGD RFCLGQLSNV HRTEAIERAR LHIGKGVQLE CKGEGDVWVR CLSDHAVFVQ SYYLDREAGR APGDAVHKIY PSAYIKVFDL RQCHRQMQQQ AATAQAAAAA QAAAVAGNIP GPGSVGGIAP AISLSAAAGI GVDDLRRLCI LRMSFVKGWG PDYPRQSIKE TPCWIEIHLH RALQLLDEVL HTMPIADPQP LDLEHHHHHH
- 产品特性
- Recombinant Human SMAD Family Member 4/SMAD4 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp552) of Human SMAD4 fused with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product SMAD4 蛋白
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SMAD Family Member 4 (SMAD4) (AA 1-552) protein (GST tag)
SMAD4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
SMAD Family Member 4 (SMAD4) (AA 13-227) protein (His tag)SMAD4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
SMAD Family Member 4 (SMAD4) (AA 56-165) protein (GST tag)SMAD4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
SMAD Family Member 4 (SMAD4) (AA 1-552) protein (Strep Tag)Crystallography grade SMAD4 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
SMAD Family Member 4 (SMAD4) (AA 1-551) protein (Strep Tag)Crystallography grade SMAD4 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
SMAD Family Member 4 (SMAD4) protein (Myc-DYKDDDDK Tag)SMAD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, pH 8.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- SMAD4 (SMAD Family Member 4 (SMAD4))
- 别名
- SMAD4 (SMAD4 产品)
- 别名
- smad4 Protein, DPC4 Protein, JIP Protein, MADH4 Protein, MYHRS Protein, AW743858 Protein, D18Wsu70e Protein, Madh4 Protein, XSmad4alpha Protein, Xsmad4 Protein, dpc4 Protein, jip Protein, madh4 Protein, xsmad4a Protein, SMAD family member 4 Protein, Smad4 protein Protein, SMAD family member 4, gene 1 L homeolog Protein, SMAD4 Protein, smad4 Protein, Smad4 Protein, smad4.1.L Protein
- Sub Type
- Fusionprotein
- 背景
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SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Alternative Names: Mothers Against Decapentaplegic Homolog 4, MAD Homolog 4, Mothers Against DPP Homolog 4, Deletion Target in Pancreatic Carcinoma 4, SMAD Family Member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4 - 分子量
- 61.5 kDa
- UniProt
- Q13485
- 途径
- Cell Division Cycle, Chromatin Binding, Autophagy
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