Optimized DNA sequence encoding Mouse extracellular domain of mouse IL-1 receptor type II including a C-terminal His tag was expressed in HEK293 cells.
产品特性
Recombinant mouse IL-1 receptor type II (CD121b) is a protein consisting of 583 amino acid residue subunits, due to glycosylation migrates as an approximately 90 kDa protein on SDS-PAGE.
纯度
> 97 %, as determined by SDS-PAGE and HPLC
过滤
0.2 μm filtered
内毒素水平
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Crystallography grade
IL1R2
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS solution, pH 7.2, 5 % Trehalose.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
-20 °C
储存方法
The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.
CD121b Protein, CDw121b Protein, IL-1R-2 Protein, IL-1RT-2 Protein, IL-1RT2 Protein, IL1R2c Protein, IL1RB Protein, Il1r-2 Protein, IL-1R2 Protein, interleukin 1 receptor type 2 Protein, interleukin 1 receptor, type II Protein, interleukin-like Protein, IL1R2 Protein, Il1r2 Protein, KK34 Protein
背景
IL11 is secreted by bone marrow stromal cells (fibroblasts)and is produced also by a number of mesenchymal cells. The IL11 receptor (alpha chain) utilizes gp130 as its signal transducer, which is also a component of other cytokine receptors. The alpha chain of the IL-11 receptor has been identified independently as Etl2. IL11 promotes primary and secondary immune responses in vitro and in vivo and modulates antigen-specific antibody reactions. IL11 promotes the proliferation of IL6 dependent plasmacytoma cell lines in the presence of neutralizing IL6 antibodies. IL11 also stimulates the T-cell dependent development of IgG-secreting B-cells in spleen cell cultures. IL11 inhibits the differentiation of pre-adipocytes. It induces the synthesis of some acute phase proteins by hepatocytes.