GAD65 Protein (AA 1-585) (GST tag)
(2 references)-
- 抗原 See all GAD65 (GAD2) 蛋白
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-585
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宿主
- 人
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资源
- 小麦胚
- 标记
- This GAD65 protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- 原理
- GAD2 (Human) Recombinant Protein (P02)
- 序列
- MASPGSGFWS FGSEDGSGDS ENPGTARAWC QVAQKFTGGI GNKLCALLYG DAEKPAESGG SQPPRAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MMIARFKMFP EVKEKGMAAL PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV PLQCSALLVR EEGLMQNCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV DVFKLWLMWR AKGTTGFEAH VDKCLELAEY LYNIIKNREG YEMVFDGKPQ HTNVCFWYIP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
- 产品特性
- Human GAD2 full-length ORF ( NP_000809.1, 1 a.a. - 585 a.a.) recombinant protein with GST-tag at N-terminal.
- 纯化方法
- in vitro wheat germ expression system
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Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) protein
GAD2 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) protein (GST tag,His tag)GAD2 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) protein (GST tag)GAD2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) protein (Strep Tag)Crystallography grade GAD2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) protein (Strep Tag)Crystallography grade GAD2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585), (full length) protein (His tag)GAD2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GAD2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - 限制
- 仅限研究用
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- 缓冲液
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Best use within three months from the date of receipt of this protein.
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: "Essential roles of insulin expression in Aire+ tolerogenic dendritic cells in maintaining peripheral self-tolerance of islet ?-cells." in: Cellular immunology, Vol. 273, Issue 2, pp. 115-23, (2012) (PubMed).
: "Thymus-specific deletion of insulin induces autoimmune diabetes." in: The EMBO journal, Vol. 28, Issue 18, pp. 2812-24, (2009) (PubMed).
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: "Essential roles of insulin expression in Aire+ tolerogenic dendritic cells in maintaining peripheral self-tolerance of islet ?-cells." in: Cellular immunology, Vol. 273, Issue 2, pp. 115-23, (2012) (PubMed).
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- 抗原
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- 别名
- GAD2 (GAD2 产品)
- 别名
- GAD65 Protein, gad65 Protein, F12P19.12 Protein, F12P19_12 Protein, GLUTAMATE DECARBOXYLASE 2 Protein, glutamate decarboxylase 2 Protein, zgc:112198 Protein, 6330404F12Rik Protein, GAD(65) Protein, Gad-2 Protein, glutamate decarboxylase 2 Protein, glutamate decarboxylase 1 Protein, glutamic acid decarboxylase 2 Protein, GAD2 Protein, PTRG_06148 Protein, LOC9324534 Protein, Gad2 Protein, GAD1 Protein, gad2 Protein
- 背景
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Full Gene Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65 kDa)
Synonyms: GAD65,MGC161605,MGC161607 - 基因ID
- 2572
- NCBI登录号
- NM_000818
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