Beclin 1 Protein (AA 1-450) (GST tag)
(3 references)-
- 抗原 See all Beclin 1 (BECN1) 蛋白
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-450
-
宿主
- 人
-
资源
- 小麦胚
- 标记
- This Beclin 1 protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- 原理
- BECN1 (Human) Recombinant Protein (P01)
- 序列
- MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK
- 产品特性
- Human BECN1 full-length ORF ( NP_003757.1, 1 a.a. - 450 a.a.) recombinant protein with GST-tag at N-terminal.
- 纯化方法
- in vitro wheat germ expression system
-
Beclin 1, Autophagy Related (BECN1) (AA 46-152) protein (His-IF2DI Tag)
BECN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 351-450) protein (GST tag)BECN1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Beclin 1, Autophagy Related (BECN1) (AA 1-450) protein (His tag)BECN1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 1-450) protein (Strep Tag)Crystallography grade BECN1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 1-448) protein (Strep Tag)Crystallography grade BECN1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Beclin 1, Autophagy Related (BECN1) (AA 150-450) protein (His tag)Crystallography grade BECN1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 134-450) protein (His tag)Crystallography grade BECN1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 132-448) protein (His tag)Crystallography grade BECN1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) (AA 148-448) protein (His tag)Crystallography grade BECN1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Beclin 1, Autophagy Related (BECN1) protein (Myc-DYKDDDDK Tag)BECN1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - 限制
- 仅限研究用
-
- 缓冲液
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -80 °C
- 储存方法
- Best use within three months from the date of receipt of this protein.
-
-
: "High Beclin 1 expression defines a poor prognosis in endometrial adenocarcinomas." in: Gynecologic oncology, Vol. 123, Issue 1, pp. 147-51, (2011) (PubMed).
: "Depletion of Beclin-1 due to proteolytic cleavage by caspases in the Alzheimer's disease brain." in: Neurobiology of disease, Vol. 43, Issue 1, pp. 68-78, (2011) (PubMed).
: "Developmentally regulated ceramide synthase 6 increases mitochondrial Ca2+ loading capacity and promotes apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4644-58, (2011) (PubMed).
-
: "High Beclin 1 expression defines a poor prognosis in endometrial adenocarcinomas." in: Gynecologic oncology, Vol. 123, Issue 1, pp. 147-51, (2011) (PubMed).
-
- 抗原
- Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
- 别名
- BECN1 (BECN1 产品)
- 别名
- beclin-1 Protein, atg6 Protein, vps30 Protein, beclin1 Protein, Beclin-1 Protein, becn1 Protein, zgc:73097 Protein, ATG6 Protein, VPS30 Protein, 4921513J16Rik Protein, 5430417M23Rik Protein, Atg6 Protein, beclin 1 Protein, beclin 1, autophagy related Protein, beclin 1 protein Protein, beclin-1 Protein, beclin family protein Protein, beclin 1, autophagy related L homeolog Protein, BECN1 Protein, becn1 Protein, atg6 Protein, CpipJ_CPIJ011087 Protein, PTRG_07825 Protein, SJAG_01876 Protein, Becn1 Protein, becn1.L Protein
- 背景
-
Full Gene Name: beclin 1, autophagy related
Synonyms: ATG6,VPS30,beclin1 - 基因ID
- 8678
- NCBI登录号
- NM_003766
- 途径
- Autophagy
-
