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TNF alpha 蛋白

Name: TNF alpha 蛋白
SKU: ABIN1305133
Availability: OutOfStock

This Recombinant TNF alpha protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 2+ publications.

(2 references)

产品编号 ABIN1305133

发货至: 中国

Quick Overview for TNF alpha 蛋白 (ABIN1305133)

抗原

See all TNF alpha 蛋白

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

蛋白类型

Recombinant

生物活性

Active

宿主

大鼠

资源

大肠杆菌（E. Coli）

应用范围

ELISA, Functional Studies (Func)

纯度

≥ 95 %

Discover our top product TNF alpha 蛋白

品牌

BD Pharmingen™

特异性

Bioassay: Activity Range Tested by MTT/Actinomycin D Cytolysis Assay using L929 indicator cells
Specific Activity: 0.25 - 5.0 x 10^9 Units /mg
ED50: 2 - 40 pg/ml
Observed Dose response: 250 fold
The Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, titrate the reagent to obtain optimal results.

产品特性

Recombinant rat TNF is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant rat TNF was purified by immunoaffinity chromatography and yielded a TNF protein fraction which was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

纯化方法

Immunoaffinity Chromatography
Recombinant rat TNF is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives. Recombinant rat TNF is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.

过滤

0.22 μm filtered

内毒素水平

The endotoxin level is ≤ 0.1 ng/µg of TNF protein, as measured in a chromogenic LAL assay.
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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)
custom-made TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)
custom-made TNF alpha 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein
HPLC verified TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 兔宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active

应用备注

ELISA Standard: Recombinant rat TNF is useful as a quantitative standard for measuring rat TNF protein levels in a TNF specific sandwich ELISA with purified TN3-19.12 antibody (Cat. No. 557516) as a capture antibody and biotinylated rabbit anti-mouse/rat TNF antibody (Cat. No. 557432) as the detection antibody. To obtain linear standard curves, doubling dilutions of rat TNF standard from ~10 ng - 0.1 ng/ml should be included in each ELISA plate.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For measuring rat TNF in serum or plasma the rat TNF OptEIA™ Set (Cat. No. 558535) and rat TNF OptEIA™ Kit (Cat. No. 560479) are specially formulated and recommended. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

说明

BD Pharmingen™ Recombinant Rat TNF - Reactivity Rat

限制

仅限研究用
状态

Liquid

浓度

100 μg/mL

缓冲液

Frozen aqueous buffered solution containing BSA.

储存液

Azide free

注意事项

Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Rat TNF is a 17.4 kD protein containing 157 amino acid residues.

储存条件

-80 °C

储存方法

Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Jäättelä: "Biologic activities and mechanisms of action of tumor necrosis factor-alpha/cachectin." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 64, Issue 6, pp. 724-42, (1991) (PubMed).

Hogan, Vogel: "Production of tumor necrosis factor by rIFN-gamma-primed C3H/HeJ (Lpsd) macrophages requires the presence of lipid A-associated proteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 12, pp. 4196-202, (1989) (PubMed).
抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF

背景

Tumor Necrosis Factor (TNF, formerly known as TNF-α) is a potent multifunctional cytokine which can exert regulatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. Rat TNF is a 17.4 kD protein containing 157 amino acid residues.

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome