Streptavidin Protein (HRP)
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- 抗原 See all Streptavidin 蛋白
- Streptavidin
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宿主
- Streptomyces avidinii
- 宿主
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- 标记
- This Streptavidin protein is labelled with HRP.
- 应用范围
- Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 品牌
- BD Pharmingen™
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- Discover our top product Streptavidin 蛋白
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Streptavidin protein (HRP)
宿主: Streptomyces avidinii WB, ELISA, IHC, DB, IF
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- 应用备注
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Immunohistochemistry: SAv-HRP is ready to use in its pre-diluted format. Remove the solution from 4°C and let it equilibrate to room temperature before using. The SAv-HRP should be used after the slides are incubated with biotinylated primary or secondary antibody and the unbound antibody is washed away. Apply enough SAv-HRP to cover the tissue sections and incubate 30 minutes at room temperature. Wash 3X with PBS and apply the appropriate substrate for horseradish peroxidase (inquire with technical service for available substrates from BD Pharmingen™).
Note: It is important not to use Azide in any of the buffers when using horseradish peroxidase as azide inactivates the enzyme. - 限制
- 仅限研究用
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- 状态
- Liquid
- 注意事项
- Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
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- 抗原
- Streptavidin
- Abstract
- Streptavidin 产品
- 背景
- Streptavidin-Horseradish peroxidase (SAv-HRP) is a useful detection enzyme for labeling biotinylated-primary or seconday antibodies for detection by immunohistochemistry. The streptavidin has very high affinity for biotin conjugated to the antibody and the horseradish peroxidase serves as the enzyme to change color in different substrates. This pre-diluted SAv-HRP is convenient to use and provides optimal signal amplification and detection. This product is routinely tested by immunohistochemistry.
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