Streptavidin Protein (Alkaline Phosphatase (AP))
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- 抗原 See all Streptavidin 蛋白
- Streptavidin
- 蛋白类型
- Native
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宿主
- Streptomyces avidinii
- 宿主
- 请咨询
- 标记
- This Streptavidin protein is labelled with Alkaline Phosphatase (AP).
- 应用范围
- Western Blotting (WB), ELISA
- 特异性
- Streptavidin Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin. Streptavidin Alkaline Phosphatase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin. No reaction was observed against anti-Avidin.
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- 应用备注
- Streptavidin Alkaline Phosphatase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).
- 说明
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Streptavidin Alkaline Phosphatase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原
- Streptavidin
- Abstract
- Streptavidin 产品
- 背景
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Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
Synonyms: ALP, Alk Phos, SA ALP - UniProt
- P22629
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