TGFB2 Protein (His tag)
(4 references)-
- 抗原 See all TGFB2 蛋白
- TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Tobacco (Nicotiana benthamiana)
- 标记
- This TGFB2 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), Immunogen (Imm), ELISA, Standard (STD)
- 序列
- HHHHHHALDA AYCFRNVQDN CCLRPLYIDF KRDLGWKWIH EPKGYNANFC AGACPYLWSS DTQHSRVLSL YNTINPEASA SPCCVSQDLE PLTILYYIGK TPKIEQLSNM IVKSCKCS
- 特异性
- Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF-beta2.
- 产品特性
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Recombinant human TGF beta 2 contains a 6-His tag at the N-terminal end, is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal free product.
Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF-beta2.
Molecular Formula: C602H909N167O171S10
Isoelectric Point: 7,72
Extinction Coefficient: E 0.1 % (1g/L) = 2.02 (A 280 nm)
This product contains no animal-derived components or impurities. It is produced by transient expression of TGF-beta3 in non-transgenic plants. - 纯化方法
- Recombinant human TGF-beta3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).
- 纯度
- > 97 % by SDS-PAGE gel
- 内毒素水平
- < 0.04 EU/µg protein (LAL method)
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Transforming Growth Factor, beta 2 (TGFB2) (AA 303-414) protein
TGFB2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Transforming Growth Factor, beta 2 (TGFB2) (AA 303-414) protein (AVI tag,Biotin)TGFB2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Transforming Growth Factor, beta 2 (TGFB2) (AA 1-414) protein (GST tag)TGFB2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transforming Growth Factor, beta 2 (TGFB2) (Active) proteinTGFB2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Transforming Growth Factor, beta 2 (TGFB2) (AA 21-302) protein (His tag)Crystallography grade TGFB2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, beta 2 (TGFB2) (AA 303-414) protein (His tag)Crystallography grade TGFB2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, beta 2 (TGFB2) (AA 303-414) protein (His tag)Crystallography grade TGFB2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transforming Growth Factor, beta 2 (TGFB2) (AA 21-302) protein (His tag)Crystallography grade TGFB2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 说明
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Human recombinant protein expressed in Nicotiana benthamiana.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng /µL. Due to the protein nature, dimmers and multimers may be observed. Upon reconstitution, It can be stored in working aliquots at -20 °C for future use.
- 浓度
- 50 ng/µL
- 缓冲液
- Tris HCl 0.05 M buffer at pH 7.4.
- 注意事项
- Reconstituted protein should be stored in working aliquots at -20 °C and it is recommended to add a carrier protein (0.1 % HSA or BSA). Repeated freezing and thawing is not recommended.
- 储存条件
- 4 °C
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: "An improved recombinant mammalian cell expression system for human transforming growth factor-beta2 and -beta3 preparations." in: Protein expression and purification, Vol. 50, Issue 1, pp. 9-17, (2006) (PubMed).
: "The transforming growth factor-beta family." in: Annual review of cell biology, Vol. 6, pp. 597-641, (1991) (PubMed).
: "Transforming growth factor-beta. A family of growth regulatory peptides." in: Annals of the New York Academy of Sciences, Vol. 593, pp. 208-17, (1990) (PubMed).
: "Identification of another member of the transforming growth factor type beta gene family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 13, pp. 4715-9, (1988) (PubMed).
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: "An improved recombinant mammalian cell expression system for human transforming growth factor-beta2 and -beta3 preparations." in: Protein expression and purification, Vol. 50, Issue 1, pp. 9-17, (2006) (PubMed).
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- 抗原
- TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
- 别名
- TGF beta 2 (TGFB2 产品)
- 别名
- LDS4 Protein, TGF-beta2 Protein, tgf-beta2 Protein, tgfb2-A Protein, BB105277 Protein, Tgf-beta2 Protein, Tgfb-2 Protein, TGF-beta 2 Protein, Tgfbr2T Protein, TGFB2 Protein, TGFbeta2 Protein, MGF Protein, TGF-B2 Protein, transforming growth factor beta 2 Protein, transforming growth factor beta 2 L homeolog Protein, transforming growth factor, beta 2 Protein, transforming growth factor, beta receptor 2 Protein, TGFB2 Protein, tgfb2.L Protein, Tgfb2 Protein, Tgfbr2 Protein, tgfb2 Protein
- 背景
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Synonyms: Transforming growth factor beta-2, TGF-beta-2
Recombinant human TGF-beta2 is a 27.08 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulphide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2 and TGF-beta3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing. - 分子量
- 27.08 kDa
- 基因ID
- 7042, 190220
- UniProt
- P61812
- 途径
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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