UBE2F Protein (His tag)
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- 抗原 See all UBE2F 蛋白
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This UBE2F protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 纯化方法
- purified by chromatography
- 纯度
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product UBE2F 蛋白
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- 说明
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Synonyms: NEDD8-conjugating enzyme UBE2F, NCE2
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- 20 mM Tris-HCl buffer, pH 8.0, 30% glycerol, 1 MM DTT, 200 mM NaCl
- 储存条件
- 4 °C
- 储存方法
- Avoid repeated freezing and thawing cycles.
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- 抗原
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- 别名
- UBE2F (UBE2F 产品)
- 别名
- NCE2 Protein, 2510010F15Rik Protein, AI851109 Protein, RGD1307608 Protein, zgc:77005 Protein, ubiquitin conjugating enzyme E2 F (putative) Protein, NEDD8-conjugating enzyme UBE2F Protein, ubiquitin-conjugating enzyme E2F (putative) Protein, ubiquitin conjugating enzyme E2F (putative) S homeolog Protein, UBE2F Protein, Tsp_01616 Protein, ube2f Protein, ube2f.S Protein, Ube2f Protein
- 背景
- UBE2F, also Known as Ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and UBE2F subfamily. Ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2F accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human UBE2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 23.7kDa (209aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_542409
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