UBE2F Protein (His tag)
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- 抗原 See all UBE2F 蛋白
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This UBE2F protein is labelled with His tag.
- 原理
- Recombinant Human UBE2F Protein (His Tag)
- 序列
- Met 1-Arg 185
- 产品特性
- A DNA sequence encoding the human UBE2F isoform 1 (Q969M7-1) (Met 1-Arg 185) was expressed, with a polyhistide tag at the N-terminus.
- 纯度
- > 94 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product UBE2F 蛋白
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Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) protein (GST tag)
UBE2F 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Ubiquitin-Conjugating Enzyme E2F (UBE2F) protein (Myc-DYKDDDDK Tag)UBE2F 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, 10 % glycerol, 2 mM DTT, pH 7.5
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- 别名
- UBE2F (UBE2F 产品)
- 别名
- NCE2 Protein, 2510010F15Rik Protein, AI851109 Protein, RGD1307608 Protein, zgc:77005 Protein, ubiquitin conjugating enzyme E2 F (putative) Protein, NEDD8-conjugating enzyme UBE2F Protein, ubiquitin-conjugating enzyme E2F (putative) Protein, ubiquitin conjugating enzyme E2F (putative) S homeolog Protein, UBE2F Protein, Tsp_01616 Protein, ube2f Protein, ube2f.S Protein, Ube2f Protein
- 背景
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Background: UBE2F is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
Synonym: NCE2
- 分子量
- 22.9 kDa
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