VCAM1 Protein (AA 25-698) (His tag)
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- 抗原 See all VCAM1 蛋白
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 25-698
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宿主
- 人
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资源
- 人细胞
- 标记
- This VCAM1 protein is labelled with His tag.
- 原理
- Recombinant Human Vascular Cell Adhesion Protein 1/VCAM-1/CD106/L1CAM (C-6His)
- 序列
- FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYS FPKDPEIHLS GPLEAGKPIT VKCSVADVYP FDRLEIDLLK GDHLMKSQEF LEDADRKSLE TKSLEVTFTP VIEDIGKVLV CRAKLHIDEM DSVPTVRQAV KELQVYISPK NTVISVNPST KLQEGGSVTM TCSSEGLPAP EIFWSKKLDN GNLQHLSGNA TLTLIAMRME DSGIYVCEGV NLIGKNRKEV ELIVQEKPFT VEISPGPRIA AQIGDSVMLT CSVMGCESPS FSWRTQIDSP LSGKVRSEGT NSTLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFP RDPEIEMSGG LVNGSSVTVS CKVPSVYPLD RLEIELLKGE TILENIEFLE DTDMKSLENK SLEMTFIPTI EDTGKALVCQ AKLHIDDMEF EPKQRQSTQT LYVNVAPRDT TVLVSPSSIL EEGSSVNMTC LSQGFPAPKI LWSRQLPNGE LQPLSENATL TLISTKMEDS GVYLCEGINQ AGRSRKEVEL IIQVTPKDIK LTAFPSESVK EGDTVIISCT CGNVPETWII LKKKAETGDT VLKSIDGAYT IRKAQLKDAG VYECESKNKV GSQLRSLTLD VQGRENNKDY FSPEVDHHHH HH
- 产品特性
- Recombinant Human Vascular Cell Adhesion Protein 1/VCAM-1 is produced by mammalian expression system in human cells. The target protein is expressed with sequence (F25-E698) of Human VCAM1 fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product VCAM1 蛋白
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Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-697) protein (His tag)
VCAM1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Vascular Cell Adhesion Molecule 1 (VCAM1) protein (Fc Tag)VCAM1 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-309) protein (His tag)VCAM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vascular Cell Adhesion Molecule 1 (VCAM1) protein (His tag)VCAM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % by SDS - PAGE SDS
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) protein (GST tag)VCAM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-123) protein (GST tag)VCAM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Cell Adhesion Molecule 1 (VCAM1) (Active) protein (His tag,Fc Tag)VCAM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Vascular Cell Adhesion Molecule 1 (VCAM1) (Active) protein (His tag)VCAM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 2 mM CaCl2, 2 mM MgCl2, 5 % Threhalose, pH 7.2.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 别名
- vcam-1 (VCAM1 产品)
- 别名
- CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein
- Sub Type
- Fusionprotein
- 背景
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VCAM-1 is a single-pass type I membrane protein, contains 7 Ig-like C2-type domains. It is an endothelial ligand for very late antigen-4 (VLA-4) and alpha4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an \endothelial cell gate\" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes."
Alternative Names: Vascular Cell Adhesion Protein 1, V-CAM 1, VCAM-1, INCAM-100, CD106, VCAM1, L1CAM - 分子量
- 75.26 kDa
- UniProt
- P19320
- 途径
- Carbohydrate Homeostasis
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