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TNF alpha Protein (AA 77-233)

Name: TNF alpha Protein (AA 77-233)
Brand: antibodies-online
SKU: ABIN1097668
Availability: OutOfStock

This Recombinant TNF alpha protein is expressed in 大肠杆菌（E. Coli）.

产品编号 ABIN1097668

发货至: 中国

Quick Overview for TNF alpha Protein (AA 77-233) (ABIN1097668)

抗原

See all TNF alpha 蛋白

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

蛋白类型

Recombinant

生物活性

Active

宿主

人

资源

大肠杆菌（E. Coli）

应用范围

Functional Studies (Func)

纯度

> 95 % as determined by reducing SDS-PAGE.

Discover our top product TNF alpha 蛋白

产品特性

AA 77-233

原理

Recombinant Human Tumor Necrosis Factor α/TNFα

序列

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

产品特性

ED50 is less than 0.03 ng/mL as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
Specific Activity of 3.0 x 10 ⁷ IU/mg.

过滤

0.2 μm filtered

内毒素水平

Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Product

表达系统

Conjugate

Origin

价格从

TNF alpha Protein (AA 1-233) (His tag)

表达系统 HEK-293 Cells

Conjugate His tag

Origin Human

价格从 136,947.45 ¥

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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)
custom-made TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)
custom-made TNF alpha 宿主: 小鼠宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein
HPLC verified TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)
TNF alpha 宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 兔宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha 宿主: 小鼠宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active

限制

仅限研究用
状态

Lyophilized

溶解方式

It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

缓冲液

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.0.

注意事项

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

储存条件

4 °C/-20 °C/-80 °C

储存方法

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

Tumor Necrosis Factor alpha/tnf-alpha

背景

Recombinant Tumor Necrosis Factor α/TNF-α is a non-glycosylated cytokine produced from E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 AA combined to form a compact bell-shaped homotrimer. The individual subunits have a relative molecular mass of 17,484 Daltons each. TNF-α is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
TNFα is a homotrimer with a subunit molecular mass of 17 kDa and plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.

分子量

17.3 kDa

UniProt

P01375

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome