DCUN1D1 Protein (AA 1-259) (His tag)
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- 抗原 See all DCUN1D1 蛋白
- DCUN1D1 (Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-259
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This DCUN1D1 protein is labelled with His tag.
- 原理
- Recombinant Human DCN1-Like Protein 1/DCUN1D1 (N-6His)
- 序列
- MGSSHHHHHH SSGLVPRGSH MNKLKSSQKD KVRQFMIFTQ SSEKTAVSCL SQNDWKLDVA TDNFFQNPEL YIRESVKGSL DRKKLEQLYN RYKDPQDENK IGIDGIQQFC DDLALDPASI SVLIIAWKFR AATQCEFSKQ EFMDGMTELG CDSIEKLKAQ IPKMEQELKE PGRFKDFYQF TFNFAKNPGQ KGLDLEMAIA YWNLVLNGRF KFLDLWNKFL LEHHKRSIPK DTWNLLLDFS TMIADDMSNY DEEGAWPVLI DDFVEFARPQ IAGTKSTTV
- 产品特性
- Recombinant Human DCN1-Like Protein 1/DCUN1D1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val259) of Human DCUN1D1 fused with a 6His tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product DCUN1D1 蛋白
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Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1) (AA 1-259) protein (His tag)
DCUN1D1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1) (AA 1-259) protein (GST tag)DCUN1D1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1) (AA 1-89) protein (GST tag)DCUN1D1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1) (AA 1-259) protein (Strep Tag)Crystallography grade DCUN1D1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1) (AA 1-259), (full length) protein (His-SUMO Tag)DCUN1D1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1) protein (Myc-DYKDDDDK Tag)DCUN1D1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- DCUN1D1 (Defective in Cullin Neddylation 1, Domain Containing 1 (DCUN1D1))
- 别名
- DCUN1D1 (DCUN1D1 产品)
- 别名
- fd19a01 Protein, zgc:66414 Protein, wu:fd19a01 Protein, dcun1l1 Protein, rp42 Protein, sccro Protein, scro Protein, tes3 Protein, DCNL1 Protein, DCUN1L1 Protein, RP42 Protein, SCCRO Protein, SCRO Protein, Tes3 Protein, Rp42 Protein, pTes3 Protein, DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) Protein, DCN1-like protein 1 Protein, DCN1, defective in cullin neddylation 1, domain containing 1 L homeolog Protein, defective in cullin neddylation 1 domain containing 1 Protein, dcun1d1 Protein, dcnl1 Protein, dcun1d1.L Protein, DCUN1D1 Protein, Dcun1d1 Protein
- Sub Type
- Fusionprotein
- 背景
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DCN1-Like Protein 1 is a protein containing 1 DCUN1 domain and 1 UBA-like domain. DCN1-Like Protein 1 contains part of an E3 Ubiquitin Ligase Complex for Neddylation. It is required for Neddylation of Cullin components of E3 Cullin-RING Ubiquitin Ligase complexes. It enhances the rate of Cullins Neddylation. DCN1-Like Protein 1 recruits the NEDD8-charged E2 Enzyme to the Cullin component. DCUN1D1 is involved in the release of inhibitory effects of CAND1 on the Cullin-RING Ligase E3 complex assembly and activity. It acts also as an oncogene facilitating malignant transformation and carcinogenic progression.
Alternative Names: DCN1-Like Protein 1, DCUN1 Domain-Containing Protein 1, Defective in Cullin Neddylation Protein 1-Like Protein 1, Squamous Cell Carcinoma-Related Oncogene, DCUN1D1, DCUN1L1, RP42, SCCRO - 分子量
- 32.3 kDa
- UniProt
- Q96GG9
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