Recombinant Human C-Type Lectin Domain Family 10 Member A/CLEC10A is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Gln61-His316) of Human CLEC10A fused with a polyhistidine tag at the C-terminus.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
Crystallography grade
CLEC10A
宿主: 小鼠
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
有效期
3 months
抗原
CLEC10A
(C-Type Lectin Domain Family 10, Member A (CLEC10A))
CLEC10A Protein, CD301 Protein, CLECSF13 Protein, CLECSF14 Protein, HML Protein, HML2 Protein, MGL Protein, CD301a Protein, M-ASGP-BP-1 Protein, Mgl Protein, Mgl1 Protein, Mgll Protein, C-type lectin domain family 10 member A Protein, C-type lectin domain containing 10A Protein, C-type lectin domain family 10, member A Protein, LOC607405 Protein, CLEC10A Protein, Clec10a Protein
Sub Type
Fusionprotein
背景
C-Type Lectin Domain Family 10 Member A (CLEC10A) is a type II transmembrane C-type lectin that is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages. CLEC10A/MGL binds and internalizes molecules with terminal nonsialylated GalNAc carbohydrates such as the Tn carcinoma antigen. CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity. It constitute a unique class of C-type lectins because of their specificity for galactose and its structural homologues. CLEC10A is thought to participate in the recognition of molecules from both altered self and pathogens due to its monosaccharide specificity for Gal and N-acetylgalactosamine (GalNAc). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Alternative Names: C-Type Lectin Domain Family 10 Member A, C-Type Lectin Superfamily Member 14, Macrophage Lectin 2, CD301, CLEC10A, CLECSF13, CLECSF14, HML