B-Cell Linker Protein (BLNK) (AA 1-456) (His tag)
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- 抗原 See all B-Cell Linker (BLNK) 蛋白
- B-Cell Linker (BLNK)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-456
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This B-Cell Linker protein is labelled with His tag.
- 原理
- Recombinant Human B-Cell Linker Protein/BLNK (C-6His)
- 序列
- MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVSLEHH HHHH
- 产品特性
- Recombinant Human B-Cell Linker Protein/BLNK is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser456) of Human BLNK with a 6His tag at the C-Terminal.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product BLNK 蛋白
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B-Cell Linker (BLNK) (AA 1-456) protein (GST tag)
BLNK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
B-Cell Linker (BLNK) (AA 301-400) protein (GST tag)BLNK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
B-Cell Linker (BLNK) (AA 1-456) protein (His tag)BLNK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.2.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- 4 °C/-20 °C/-80 °C
- 储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - 有效期
- 3 months
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- 抗原
- B-Cell Linker (BLNK)
- 别名
- B-Cell Linker Protein/BLNK (BLNK 产品)
- 别名
- BLNK Protein, blnk Protein, MGC147045 Protein, BASH Protein, Bca Protein, Ly-57 Protein, Ly57 Protein, Lyw-57 Protein, SLP-65 Protein, AGM4 Protein, BLNK-S Protein, LY57 Protein, SLP65 Protein, bca Protein, B-cell linker Protein, B cell linker Protein, BLNK Protein, blnk Protein, Blnk Protein
- Sub Type
- Fusionprotein
- 背景
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B-Cell Linker Protein (BLNK) is a cell membrane protein which contains 1 SH2 domain. BLNK is expressed in B cells and fibroblast cell lines, playing a important role in B cell receptor signaling. BLNK as a central linker protein, downstream of the B-cell receptor (BCR), bridges the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. BLNK associates with the activation of ERK/EPHB2, MAP kinase p38 and JNK, modulates AP1, NF-kappa-B and NFAT activation. BLNK involves in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK deficiency results in agammaglobulinemia type 4 and much more profound block in B-cell development.
Alternative Names: B-Cell Linker Protein, B-Cell Adapter Containing a SH2 Domain Protein, B-Cell Adapter Containing a Src Homology 2 Domain Protein, Cytoplasmic Adapter Protein, Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa, SLP-65, BLNK, BASH, SLP65 - 分子量
- 51.5 kDa
- UniProt
- Q8WV28
- 途径
- BCR Signaling
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