B-Cell Linker Protein (BLNK) (His tag)
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- 抗原 See all B-Cell Linker (BLNK) 蛋白
- B-Cell Linker (BLNK)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This B-Cell Linker protein is labelled with His tag.
- 原理
- Recombinant Human BLNK/Ly-57 Protein (His Tag)(Active)
- 序列
- Met 1-Ser 456
- 产品特性
- A DNA sequence encoding the human BLNK isoform 1 (AAH18906.1) (Met 1-Ser 456) was fused with a polyhistidine tag at the N-terminus.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to bind human BTK in a functional ELISA.
- Top Product
- Discover our top product BLNK 蛋白
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B-Cell Linker (BLNK) (AA 1-456) protein (GST tag)
BLNK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
B-Cell Linker (BLNK) (AA 301-400) protein (GST tag)BLNK 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
B-Cell Linker (BLNK) (AA 1-456) protein (His tag)BLNK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
B-Cell Linker (BLNK) (AA 1-456) protein (Strep Tag)Crystallography grade BLNK 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
B-Cell Linker (BLNK) (AA 1-457) protein (Strep Tag)Crystallography grade BLNK 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- B-Cell Linker (BLNK)
- 别名
- BLNK/Ly-57 (BLNK 产品)
- 别名
- BLNK Protein, blnk Protein, MGC147045 Protein, BASH Protein, Bca Protein, Ly-57 Protein, Ly57 Protein, Lyw-57 Protein, SLP-65 Protein, AGM4 Protein, BLNK-S Protein, LY57 Protein, SLP65 Protein, bca Protein, B-cell linker Protein, B cell linker Protein, BLNK Protein, blnk Protein, Blnk Protein
- 背景
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Background: B-cell linker protein, also known as B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65 and BLNK, is a cytoplasm and cell membrane protein which contains one SH2 domain. BLNK is expressed in B-cell lineage and fibroblast cell lines. Highest levels of expression is in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. BLNK functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. BLNK plays a role in the activation of ERK / EPHB2, MAP kinase p38 and JNK. BLNK modulates AP1 activation. It is important for the activation of NF-kappa-B and NFAT. BLNK plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. BLNK may be required for the RAC1-JNK pathway. It plays a critical role in orchestrating the pro-B cell to pre-B cell transition. BLNK also plays an important role in BCR-induced B-cell apoptosis. Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) which is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development.
Synonym: B-Cell Linker Protein; B-Cell Adapter Containing a SH2 Domain Protein; B-Cell Adapter Containing a Src Homology 2 Domain Protein; Cytoplasmic Adapter Protein; Src Homology 2 Domain-Containing Leukocyte Protein of 65 kDa; SLP-65; BLNK; BASH; SLP65
- 分子量
- 53 kDa
- 途径
- BCR Signaling
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