NGFB Protein (AA 122-241)
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Quick Overview for NGFB Protein (AA 122-241) (ABIN1096128)
抗原
See all NGFB 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 122-241
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原理
- Recombinant Human β-Nerve Growth Factor/β-NGF (Ser122-Ala241, E. coli)
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序列
- SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
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产品特性
- Recombinant Human NGF Beta (beta-Nerve Growth Factor/beta-NGF) produced in E. coli is a non-glycosylated non-covalent homodimer of two 118 amino acid polypeptides each with a molecular mass of 13.4 kDa.
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Nerve Growth Factor beta (NGFB) (Active) protein
NGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Nerve Growth Factor beta (NGFB) proteinNGFB 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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说明
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Biological activity: ED50 is less than 1.0 ng/ml as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.Specific Activity is greater than 1 x 106 IU/mg.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.0.
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注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
有效期
- 3 months
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- NGFB (Nerve Growth Factor beta (NGFB))
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别名
- beta-Nerve Growth Factor
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背景
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Recombinant Human NGF Beta: beta-Nerve Growth Factor/beta-NGF was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits alpha, beta, and gamma, it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Beta-NGF is a neurotrophic factor that signals through its receptor beta-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that beta-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Recombinant Human NGF Beta shares 90% sequence similarity with mouse protein and shows cross-species reactivity.ReferencesForsell P, et al. The use of TrkA-PathHunter assay in high-throughput screening to identify compounds that affect nerve growth factor signaling.PMID: 23458757http://www.ncbi.nlm.nih.gov/pubmed/23458757
Alternative Names: Recombinant Human NGF Beta, Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB -
分子量
- 13.4 kDa
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UniProt
- P01138
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途径
- NF-kappaB Signaling, RTK signaling, Regulation of Cell Size
抗原
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